Identification of SNPs in Cellular Retinol Binding Protein 1 and Cellular Retinol Binding Protein 3 Genes and Their Associations with Laying Performance Traits in Erlang Mountainous Chicken.
Identification of SNPs in Cellular Retinol Binding Protein 1 and Cellular Retinol Binding Protein 3 Genes and Their Associations with Laying Performance Traits in Erlang Mountainous Chicken.
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DOI：
10.5713/ajas.2013.13587
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Zhu Q
中科院分区：
文献类型：
--
作者：
Wang Y;Xiao LH;Zhao XL;Liu YP;Zhu Q
CRBP1(细胞视黄醇结合蛋白1)和CRBP3(细胞视黄醇结合蛋白3)是视黄醇信号通路的重要组成部分，参与维生素A的吸收、转运和代谢。本研究基于维生素A在蛋鸡生产性能中的作用，采用单链构象多态性和DNA测序的方法，对349只鸡的CRBP1和CRBP3基因的多态性进行了研究。CRBP1基因第三内含子只有一个多态性，CRBP3基因有两个多态性，分别位于第二内含子和第三内含子。对这三个SNP与二郎山鸡产蛋性能的相关性进行了研究。值得注意的是，CRBP1的SNP g.14604G>；T与初产蛋体重(BWFE)、初产蛋龄(AFE)、初产蛋重(WFE)和300日龄产蛋总数(EN)显著相关。CRBP3基因g.934C和gt；G与AFE相关，g.1324A和gt；G与AFE和BWFE相关，但与鸡蛋品质性状无关联。在CRBP3基因的这两个SNP上构建的单倍型组合与BWFE和AFE相关。其中，双倍型H_2H_2对AFE、BWFE、EN和平均产蛋间隔有正向影响。在此，我们首次对鸡CRBP1和CRBP3基因的多态性进行了基础研究，这些基因的多态性可以预测鸡的产蛋性能。
CRBP1 (cellular retinol binding protein 1) and CRBP3 (cellular retinol binding protein 3), are important components of the retinoid signaling pathway and take part in vitamin A absorption, transport and metabolism. Based on the role of vitamin A in chicken laying performance, we investigated the polymorphism of CRBP1 and CRBP3 genes in 349 chickens using single strand conformation polymorphism and DNA sequencing methods. Only one polymorphism was identified in the third intron of CRBP1, two polymorphisms were detected in CRBP3; they were located in the second intron and the third intron respectively. The association studies between these three SNPs and laying performance traits were performed in Erlang mountainous chicken. Notably, the SNP g.14604G>T of CRBP1 was shown to be significantly associated with body weight at first egg (BWFE), age at first egg (AFE), weight at first egg (WFE) and total number of eggs with 300 age (EN). The CRBP3 polymorphism g.934C>G was associated with AFE, and the g.1324A>G was associated with AFE and BWFE, but none of these polymorphisms were associated with egg quality traits. Haplotype combinations constructed on these two SNPs of CRBP3 gene were associated with BWFE and AFE. In particular, diplotype H2H2 had positive effect on AFE, BWFE, EN, and average egg-laying interval. We herein describe for the first time basic research on the polymorphism of chicken CRBP1 and CRBP3 genes that is predictive of genetic potential for laying performance in chicken.
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影响因子：
4.4
作者：
Lin, H;Wang, LF;Yang, QM
通讯作者：
Yang, QM
DOI：
10.1016/j.bbalip.2011.06.002
发表时间：
2012-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
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通讯作者：
Harrison EH
影响因子：
3.5
作者：
Capon, F;Allen, MH;Trembath, RC
通讯作者：
Trembath, RC
影响因子：
4.2
作者：
NAPOLI, JL
通讯作者：
NAPOLI, JL
影响因子：
30.8
作者：
Daly, MJ;Rioux, JD;Lander, ES
通讯作者：
Lander, ES