A microfluidic system for studying ageing and dynamic single-cell responses in budding yeast.
A microfluidic system for studying ageing and dynamic single-cell responses in budding yeast.
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DOI：
10.1371/journal.pone.0100042
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Swain PS
中科院分区：
文献类型：
--
作者：
Crane MM;Clark IB;Bakker E;Smith S;Swain PS
细胞间变异性在细胞决策中的重要性的认识以及对细胞过程随机建模的日益增长的兴趣导致了对在时变环境中的高密度、可重复的单细胞测量的需求的增加。我们提出了ALCATRAS(一种长期培养和捕获系统)，这是一种微流控设备，可以在定义良好和可控的环境中定量监测多达1000个萌芽酵母细胞。子代细胞通过流体流动移走，以避免拥挤，允许实验运行60多个小时，细胞外培养液可能会在几秒钟内反复更换。我们通过复制寿命曲线测量老化，跟踪细胞周期的动态，并检查一般应激反应中依赖历史的行为，来说明该设备的使用。
Recognition of the importance of cell-to-cell variability in cellular decision-making and a growing interest in stochastic modeling of cellular processes has led to an increased demand for high density, reproducible, single-cell measurements in time-varying surroundings. We present ALCATRAS (A Long-term Culturing And TRApping System), a microfluidic device that can quantitatively monitor up to 1000 cells of budding yeast in a well-defined and controlled environment. Daughter cells are removed by fluid flow to avoid crowding allowing experiments to run for over 60 hours, and the extracellular media may be changed repeatedly and in seconds. We illustrate use of the device by measuring ageing through replicative life span curves, following the dynamics of the cell cycle, and examining history-dependent behaviour in the general stress response.
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影响因子：
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