Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells.
Rice OsBRCA2 Is Required for DNA Double-Strand Break Repair in Meiotic Cells.
复制标题
水稻OsBRCA2是减数分裂细胞DNA双链断裂修复所必需的。
DOI：
10.3389/fpls.2020.600820
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发表时间：
2020
影响因子：
5.6
通讯作者：
Liang W
中科院分区：
文献类型：
--
作者：
Fu R;Wang C;Shen H;Zhang J;Higgins JD;Liang W
哺乳动物乳腺癌2 （BRCA2）基因是一种肿瘤抑制因子，在DNA修复和同源重组（HR）中起重要作用。在这里，我们报告了人类BRCA2的水稻同源物OsBRCA2的鉴定和表征。Osbrca2突变体植物表现出正常的营养生长，但由于严重的减数分裂缺陷而经历完全的雄性和雌性不育。配对、突触和重组在osbrca2雄性减数细胞中受损，导致染色体缠结和断裂。在缺乏OsBRCA2的情况下，链入侵蛋白OsRAD51和OsDMC1在减数分裂染色体轴上的定位严重减少，并且在体外，OsBRCA2直接与OsRAD51和OsDMC1相互作用。这些结果表明，在减数分裂期间，OsBRCA2对于促进OsRAD51和OsDMC1加载到程序性双链断裂（DSB）的切除端以促进同源染色体的单端入侵和准确重组至关重要。此外，用丝裂霉素C （MMC）处理osbrca2-1幼苗导致过敏。由于MMC是一种基因毒性物质，在体细胞中产生DNA损伤，只能通过HR修复，这些结果表明OsBRCA2在水稻DSB修复和HR中具有保守作用。
The mammalian BREAST CANCER 2 (BRCA2) gene is a tumor suppressor that plays a crucial role in DNA repair and homologous recombination (HR). Here, we report the identification and characterization of OsBRCA2, the rice orthologue of human BRCA2. Osbrca2 mutant plants exhibit normal vegetative growth but experience complete male and female sterility as a consequence of severe meiotic defects. Pairing, synapsis and recombination are impaired in osbrca2 male meiocytes, leading to chromosome entanglements and fragmentation. In the absence of OsBRCA2, localization to the meiotic chromosome axes of the strand-invasion proteins OsRAD51 and OsDMC1 is severely reduced and in vitro OsBRCA2 directly interacts with OsRAD51 and OsDMC1. These results indicate that OsBRCA2 is essential for facilitating the loading of OsRAD51 and OsDMC1 onto resected ends of programmed double-strand breaks (DSB) during meiosis to promote single-end invasions of homologous chromosomes and accurate recombination. In addition, treatment of osbrca2-1 seedlings with mitomycin C (MMC) led to hypersensitivity. As MMC is a genotoxic agent that creates DNA lesions in the somatic cells that can only be repaired by HR, these results suggest that OsBRCA2 has a conserved role in DSB repair and HR in rice.
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影响因子：
3.7
作者：
Morozumi Y;Ino R;Ikawa S;Mimida N;Shimizu T;Toki S;Ichikawa H;Shibata T;Kurumizaka H
通讯作者：
Kurumizaka H
影响因子：
4.8
作者：
Chen, CF;Chen, PL;Lee, WH
通讯作者：
Lee, WH
DOI：
10.1126/science.1219379
发表时间：
2012-09-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Cloud V;Chan YL;Grubb J;Budke B;Bishop DK
通讯作者：
Bishop DK
影响因子：
2.7
作者：
Collins, Tony J.
通讯作者：
Collins, Tony J.
影响因子：
3.5
作者：
Hussain, S;Witt, E;Mathew, CG
通讯作者：
Mathew, CG