Muscle on a chip: in vitro contractility assays for smooth and striated muscle.
Muscle on a chip: in vitro contractility assays for smooth and striated muscle.
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DOI：
10.1016/j.vascn.2012.04.001
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发表时间：
2012-05
影响因子：
1.9
通讯作者：
Parker KK
中科院分区：
文献类型：
--
作者：
Grosberg A;Nesmith AP;Goss JA;Brigham MD;McCain ML;Parker KK
为了评估肌肉组织的活力，测量组织的收缩性能以及控制其结构是必不可少的。准确的收缩力数据可以帮助开发更有效和更安全的药物。这可以通过适用于各种类型肌肉组织的强大的体外收缩性测定来完成。在这项工作中开发的设备是基于肌肉薄膜（MTF）技术，其中在一侧用二维工程肌肉组织制造弹性薄膜。组织模板是用微接触印刷技术对细胞外基质进行图案化制备的。当肌肉细胞被植入薄膜时，它们会根据基质中的几何线索自我组织，形成组织。基于“芯片上MTF”技术的几种分析方法进行了演示。一种这样的试验将横纹肌的收缩性试验并入流体通道中。另一种分析平台将mtf集成在多孔板中，与自动数据收集和分析兼容。最后，我们证明了在同一芯片上同时分析横纹肌和平滑肌收缩性的可能性。在这项工作中，我们组装了一套基于肌肉薄膜的横纹肌和平滑肌收缩性分析。我们的结果表明，在目前的方法和替代分离组织制备的改进。我们的技术适用于原代收获细胞和细胞系，以及人类和动物组织。
To evaluate the viability of a muscle tissue, it is essential to measure the tissue’s contractile performance as well as to control its structure. Accurate contractility data can aid in development of more effective and safer drugs. This can be accomplished with a robust in vitro contractility assay applicable to various types of muscle tissue. The devices developed in this work were based on the muscular thin film (MTF) technology, in which an elastic film is manufactured with a 2D engineered muscle tissue on one side. The tissue template is made by patterning extracellular matrix with microcontact printing. When muscle cells are seeded on the film, they self-organize with respect to the geometric cues in the matrix to form a tissue. Several assays based on the “MTF on a chip” technology are demonstrated. One such assay incorporates the contractility assay with striated muscle into a fluidic channel. Another assay platform incorporates the MTFs in a multi-well plate, which is compatible with automated data collection and analysis. Finally, we demonstrate the possibility of analyzing contractility of both striated and smooth muscle simultaneously on the same chip. In this work, we assembled an ensemble of contractility assays for striated and smooth muscle based on muscular thin films. Our results suggest an improvement over current methods and an alternative to isolated tissue preparations. Our technology is amenable to both primary harvests cells and cell lines, as well as both human and animal tissues.
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影响因子：
--
作者：
Bray, Mark-Anthony;Sheehy, Sean P.;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
DOI：
10.1016/j.vascn.2004.02.007
发表时间：
2004-05-01
影响因子：
1.9
作者：
Bass, Alan;Kinter, Lewis;Williams, Patricia
通讯作者：
Williams, Patricia
影响因子：
6.1
作者：
Grosberg A;Alford PW;McCain ML;Parker KK
通讯作者：
Parker KK
影响因子：
14
作者：
Alford, Patrick W.;Feinberg, Adam W.;Sheehy, Sean P.;Parker, Kevin K.
通讯作者：
Parker, Kevin K.
DOI：
10.1007/s10820-006-9045-6
发表时间：
2007-04-01
期刊：
JOURNAL OF COMPUTER-AIDED MATERIALS DESIGN
影响因子：
--
作者：
Adams, W. J.;Pong, T.;Parker, K. K.
通讯作者：
Parker, K. K.