Ensembles of engineered cardiac tissues for physiological and pharmacological study: heart on a chip.
Ensembles of engineered cardiac tissues for physiological and pharmacological study: heart on a chip.
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DOI：
10.1039/c1lc20557a
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发表时间：
2011-12-21
期刊：
影响因子：
6.1
通讯作者：
Parker KK
中科院分区：
文献类型：
--
作者：
Grosberg A;Alford PW;McCain ML;Parker KK
传统上，肌肉生理学实验需要多个组织样品才能获得形态学，电生理学和收缩性数据。案例，样品的变异性阻碍了实验组之间的定量比较。肌肉薄膜技术 - 弹性体薄膜上工程性的，各向异性的心室心肌的生物杂化构建体 - 测量收缩性，并在同一实验中多次对动作电位传播的量化和细胞骨架结构。药物干预期间的时间数据收集和分析。这复制了层状心脏肌肉的分层组织结构。
Traditionally, muscle physiology experiments require multiple tissue samples to obtain morphometric, electrophysiological, and contractility data. Furthermore, these experiments are commonly completed one at a time on cover slips of single cells, isotropic monolayers, or in isolated muscle strips. In all of these cases, variability of the samples hinders quantitative comparisons among experimental groups. Here, we report the design of a “heart on a chip” that exploits muscular thin film technology – biohybrid constructs of an engineered, anisotropic ventricular myocardium on an elastomeric thin film – to measure contractility, combined with a quantification of action potential propagation, and cytoskeletal architecture in multiple tissues in the same experiment. We report techniques for real-time data collection and analysis during pharmacological intervention. The chip is an efficient means of measuring structure-function relationships in constructs that replicate the hierarchical tissue architectures of laminar cardiac muscle.
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影响因子：
--
作者：
Bray, Mark-Anthony;Sheehy, Sean P.;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
5.5
作者：
Fedorov, Vadim V.;Lozinsky, Ilya T.;Efimov, Igor R.
通讯作者：
Efimov, Igor R.
DOI：
10.1109/34.709565
发表时间：
1998-08-01
影响因子：
23.6
作者：
Hong, L;Wan, YF;Jain, A
通讯作者：
Jain, A
影响因子：
14
作者：
Entcheva, E;Bien, H;Kostov, Y
通讯作者：
Kostov, Y
影响因子：
20.1
作者：
Gillis, AM;Fast, VG;Kleber, AG
通讯作者：
Kleber, AG