Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain.
Profile of gene expression changes during estrodiol-17β-induced feminization in the Takifugu rubripes brain.
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雌二醇 17β 诱导 Takifugu rubripes 大脑女性化过程中基因表达变化的概况
DOI：
10.1186/s12864-021-08158-0
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发表时间：
2021-11-24
期刊：
影响因子：
4.4
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Shen X;Yan H;Jiang J;Li W;Xiong Y;Liu Q;Liu Y
背景
脑作为中枢神经系统的关键组织，已被发现与性腺发育有关。此前的研究表明，性腺的命运可能会受到大脑的影响。在雌二醇(estrodiol-17β，E_2)诱导的雌性化过程中，识别大脑特有的分子变化对于我们理解鱼类大脑对性别分化的分子控制至关重要。
结果
本研究比较了雌二醇处理55天后红河豚幼虫脑内的差异转录反应。结果表明，E_2处理-XX(E-XX)和对照-XX(C-XX)之间有514个基因差异表达，而E-XY(E-XY)和对照XY(C-XY)之间有362个基因差异表达。在雌雄动物中，Cyp19a1b、GnRh 1和Pgr的表达在E2处理后显著上调，而生长激素/催乳素家族中的st、sl、Tshβ、Prl和Pit-1的表达在雌雄两性中均显著下调。参与昼夜节律的arntl1、bhlbe、nr1d2、per1b、per3、cry1、cipc和ciart基因也发生了改变。在E-XX和C-XX之间发现的差异表达基因(DEG)在神经活性配体-受体相互作用、花生四烯酸代谢、细胞因子-细胞因子受体相互作用和钙信号通路等方面显著丰富。E-XY和C-XY之间的DEG显著富含酪氨酸代谢、苯丙氨酸代谢、花生四烯酸代谢和亚油酸代谢。
结论
用RNA-seq方法在经E2处理的红色毛滴虫幼虫的脑中发现了一些基因和途径。这为进一步研究脑-垂体-性腺轴网络在红毛虫性别分化中的可能参与提供了机会。
Background
As the critical tissue of the central nervous system, the brain has been found to be involved in gonad development. Previous studies have suggested that gonadal fate may be affected by the brain. Identifying brain-specific molecular changes that occur during estrodiol-17β (E2) -induced feminization is crucial to our understanding of the molecular control of sex differentiation by the brains of fish.
Results
In this study, the differential transcriptomic responses of the Takifugu rubripes larvae brain were compared after E2 treatment for 55 days. Our results showed that 514 genes were differentially expressed between E2-treated-XX (E-XX) and Control-XX (C-XX) T. rubripes, while 362 genes were differentially expressed between E2-treated-XY (E-XY) and Control-XY (C-XY). For example, the expression of cyp19a1b, gnrh1 and pgr was significantly up-regulated, while st, sl, tshβ, prl and pit-1, which belong to the growth hormone/prolactin family, were significantly down-regulated after E2 treatment, in both sexes. The arntl1, bhlbe, nr1d2, per1b, per3, cry1, cipc and ciart genes, which are involved in the circadian rhythm, were also found to be altered. Differentially expressed genes (DEGs), which were identified between E-XX and C-XX, were significantly enriched in neuroactive ligand-receptor interaction, arachidonic acid metabolism, cytokine-cytokine receptor interaction and the calcium signaling pathway. The DEGs that were identified between E-XY and C-XY were significantly enriched in tyrosine metabolism, phenylalanine metabolism, arachidonic acid metabolism and linoleic acid metabolism.
Conclusion
A number of genes and pathways were identified in the brain of E2-treated T. rubripes larvae by RNA-seq. It provided the opportunity for further study on the possible involvement of networks in the brain-pituitary-gonadal axis in sex differentiation in T. rubripes.
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影响因子：
3.6
作者：
Davis, Lori K.;Hiramatsu, Naoshi;Grau, Gordon
通讯作者：
Grau, Gordon
DOI：
10.1016/s0169-328x(03)00339-5
发表时间：
2003-10-21
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Dewing, P;Shi, T;Vilain, E
通讯作者：
Vilain, E
影响因子：
3.1
作者：
CHEVASSUS, B;DEVAUX, A;JALABERT, B
通讯作者：
JALABERT, B
影响因子：
5.3
作者：
Adams, Caroline;Stroberg, Wylie;Moenter, Suzanne M.
通讯作者：
Moenter, Suzanne M.
影响因子：
3.5
作者：
Han, YS;Liao, IC;Yu, JYL
通讯作者：
Yu, JYL