Success and failure rates of tumor genotyping techniques in routine pathological samples with non-small-cell lung cancer.
Success and failure rates of tumor genotyping techniques in routine pathological samples with non-small-cell lung cancer.
复制标题
DOI：
10.1016/j.lungcan.2014.01.013
复制
发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Costa DB
中科院分区：
文献类型：
--
作者：
Vanderlaan PA;Yamaguchi N;Folch E;Boucher DH;Kent MS;Gangadharan SP;Majid A;Goldstein MA;Huberman MS;Kocher ON;Costa DB
非小细胞肺癌（NSCLC）中某些体细胞分子改变的鉴定已成为循证实践。在日常NSCLC病理学样本中使用市售肿瘤基因分型技术的成功率和失败率尚未得到充分描述。我们试图在接受常规临床肿瘤基因型的肺癌队列中评估EGFR突变、KRAS突变和ALK FISH的成功率和失败率。回顾性收集和分析了381例患者肿瘤样本的临床病理资料、肿瘤基因型成功率和失败率。在这381例肺癌患者中，平均年龄为65岁，61.2%为女性，75.9%为白色，27.8%从不吸烟，73.8%为晚期NSCLC，86.1%为腺癌组织学。48.8%的肿瘤组织来自手术标本，17.9%来自空芯针活检，33.3%来自抽吸物或液体的细胞块。组织采集的解剖部位包括肺（49.3%）、淋巴结（22.3%）、胸膜（11.8%）、骨（6.0%）、脑（6.0%）等。EGFR突变分析的总体成功率为94.2%，KRAS突变为91.6%，ALK FISH为91.6%。从图像引导下经皮经胸芯针活检（EGFR、KRAS和ALK检测分别为31.8%、27.3%和35.3%）和骨标本（分别为23.1%、15.4%和23.1%）中获得的组织失败率最高。在骨标本中，活检标本的失败率显著高于切除标本（EGFR为40% vs 0%，p=0.024），脱钙标本的失败率显著高于非脱钙标本（EGFR为60% vs 5.5%，p=0.021）。肿瘤基因分型技术在大多数样本中是可行的，除了小的图像引导下经皮经胸芯针活检和脱钙芯活检的骨样本，因此将常规肿瘤基因分型扩展到NSCLC患者的护理中可能不需要特殊的组织采集或操作。
Identification of some somatic molecular alterations in non-small-cell lung cancer (NSCLC) has become evidence-based practice. The success and failure rate of using commercially-available tumor genotyping techniques in routine day-to-day NSCLC pathology samples is not well described. We sought to evaluate the success and failure rate of EGFR mutation, KRAS mutation, and ALK FISH in a cohort of lung cancers subjected to routine clinical tumor genotype. Clinicopathologic data, tumor genotype success and failure rates were retrospectively compiled and analyzed from 381 patient-tumor samples. From these 381 patients with lung cancer, the mean age was 65 years, 61.2% were women, 75.9% were white, 27.8% were never smokers, 73.8% had advanced NSCLC and 86.1% had adenocarcinoma histology. The tumor tissue was obtained from surgical specimens in 48.8%, core needle biopsies in 17.9%, and as cell blocks from aspirates or fluid in 33.3% of cases. Anatomic sites for tissue collection included lung (49.3%), lymph nodes (22.3%), pleura (11.8%), bone (6.0%), brain (6.0%), among others. The overall success rate for EGFR mutation analysis was 94.2%, for KRAS mutation 91.6% and for ALK FISH 91.6%. The highest failure rates were observed when the tissue was obtained from image-guided percutaneous transthoracic core-needle biopsies (31.8%, 27.3%, and 35.3% for EGFR, KRAS, and ALK tests, respectively) and bone specimens (23.1%, 15.4%, and 23.1%, respectively). In specimens obtained from bone, the failure rates were significantly higher for biopsies than resection specimens (40% vs 0%, p=0.024 for EGFR) and for decalcified compared to non-decalcified samples (60% vs 5.5%, p=0.021 for EGFR). Tumor genotype techniques are feasible in most samples, outside small image-guided percutaneous transthoracic core-needle biopsies and bone samples from core biopsies with decalcification, and therefore expansion of routine tumor genotype into the care of patients with NSCLC may not require special tissue acquisition or manipulation.
登录
查看更多内容
DOI：
10.1158/1078-0432.ccr-08-1455
发表时间：
2008-11-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Costa DB;Nguyen KS;Cho BC;Sequist LV;Jackman DM;Riely GJ;Yeap BY;Halmos B;Kim JH;Jänne PA;Huberman MS;Pao W;Tenen DG;Kobayashi S
通讯作者：
Kobayashi S
表皮生长因子受体和KRAS突变对以前未经治疗的非小细胞肺癌患者的临床结果的影响：临床试验的在线肿瘤注册表的结果。
DOI：
10.1158/1078-0432.ccr-09-0888
发表时间：
2009-08-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Jackman DM;Miller VA;Cioffredi LA;Yeap BY;Jänne PA;Riely GJ;Ruiz MG;Giaccone G;Sequist LV;Johnson BE
通讯作者：
Johnson BE
影响因子：
4.6
作者：
Travis WD;Brambilla E;Noguchi M;Nicholson AG;Geisinger K;Yatabe Y;Ishikawa Y;Wistuba I;Flieder DB;Franklin W;Gazdar A;Hasleton PS;Henderson DW;Kerr KM;Petersen I;Roggli V;Thunnissen E;Tsao M
通讯作者：
Tsao M
影响因子：
45.3
作者：
Keedy, Vicki Leigh;Temin, Sarah;Giaccone, Giuseppe
通讯作者：
Giaccone, Giuseppe
影响因子：
254.7
作者：
Siegel, Rebecca;Naishadham, Deepa;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin