Radiation attenuates physiological angiogenesis by differential expression of VEGF, Ang-1, tie-2 and Ang-2 in rat brain.
Radiation attenuates physiological angiogenesis by differential expression of VEGF, Ang-1, tie-2 and Ang-2 in rat brain.
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DOI：
10.1667/rr2647.1
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发表时间：
2011-12
影响因子：
3.4
通讯作者：
Lee YW
中科院分区：
文献类型：
--
作者：
Lee WH;Cho HJ;Sonntag WE;Lee YW
放射性脑血管稀疏的病因尚不清楚。在本研究中，我们研究了全脑照射对大鼠脑内内皮细胞（EC）增殖/凋亡和各种血管生成因子表达的影响。F344×BN大鼠接受单次10戈伊γ射线全脑照射或假照射，并在照射后4、8和24 h维持。采用免疫荧光双染法观察脑内EC增殖/凋亡情况。实时荧光定量RT-PCR和免疫荧光染色检测脑组织血管内皮生长因子（VEGF）、血管生成素-1（Ang-1）、内皮特异性受体酪氨酸激酶（Tie-2）和Ang-2的mRNA和蛋白表达水平。与假照射对照组相比，在照射大鼠脑中检测到CD 31免疫反应阳性细胞显着减少。全脑照射显著抑制EC增殖，增加EC凋亡。此外，VEGF，Ang-1和Tie-2的mRNA和蛋白表达的显着下降，观察到在照射大鼠脑。相反，全脑照射显著上调大鼠脑中Ang-2的表达。本研究提供了新的证据表明，全脑照射差异影响VEGF，Ang-1，Tie-2和Ang-2的mRNA和蛋白质表达。这些变化与脑内EC增殖减少和EC凋亡增加密切相关。
The etiology of radiation-induced cerebrovascular rare-faction remains unknown. In the present study, we examined the effect of whole-brain irradiation on endothelial cell (EC) proliferation/apoptosis and expression of various angiogenic factors in rat brain. F344×BN rats received either whole-brain irradiation (a single dose of 10 Gy γ rays) or sham irradiation and were maintained for 4, 8 and 24 h after irradiation. Double immunofluorescence staining was employed to visualize EC proliferation/apoptosis in brain. The mRNA and protein expression levels of vascular endothelial growth factor (VEGF), angiopoietin-1 (Ang-1), endothelial-specific receptor tyrosine kinase (Tie-2), and Ang-2 in brain were determined by real-time RT-PCR and immunofluores-cence staining. A significant reduction in CD31-immunore-active cells was detected in irradiated rat brains compared with sham-irradiated controls. Whole-brain irradiation significantly suppressed EC proliferation and increased EC apoptosis. In addition, a significant decrease in mRNA and protein expression of VEGF, Ang-1 and Tie-2 was observed in irradiated rat brains. In contrast, whole-brain irradiation significantly upregulated Ang-2 expression in rat brains. The present study provides novel evidence that whole-brain irradiation differentially affects mRNA and protein expression of VEGF, Ang-1, Tie-2 and Ang-2. These changes are closely associated with decreased EC proliferation and increased EC apoptosis in brain.
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辐照引起大鼠脑促炎环境的区域特异性改变。
影响因子：
2.6
作者：
Lee, Won Hee;Sonntag, William E.;Mitschelen, Matthew;Yan, Han;Lee, Yong Woo
通讯作者：
Lee, Yong Woo
影响因子：
3.1
作者：
Deng, XQ;Li, HJ;Tang, YW
通讯作者：
Tang, YW
DOI：
10.1016/j.ijrobp.2010.12.032
发表时间：
2012-04-01
影响因子：
7
作者：
Lee, Won Hee;Warrington, Junie P.;Sonntag, William E.;Lee, Yong Woo
通讯作者：
Lee, Yong Woo
DOI：
10.1016/0360-3016(94)00332-f
发表时间：
1995-01-01
影响因子：
7
作者：
LAMPROGLOU, I;CHEN, QM;DELATTRE, JY
通讯作者：
DELATTRE, JY
影响因子：
56.9
作者：
Alavi, A;Hood, JD;Cheresh, DA
通讯作者：
Cheresh, DA