Can stem cells be used to generate new lungs? Ex vivo lung bioengineering with decellularized whole lung scaffolds.
Can stem cells be used to generate new lungs? Ex vivo lung bioengineering with decellularized whole lung scaffolds.
复制标题
DOI：
10.1111/resp.12102
复制
发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Weiss DJ
中科院分区：
文献类型：
--
作者：
Wagner DE;Bonvillain RW;Jensen T;Girard ED;Bunnell BA;Finck CM;Hoffman AM;Weiss DJ
对于终末期肺部疾病患者，肺移植是唯一可用的治疗选择。然而，合适的供肺数量不足，肺移植合并严重的移植物衰竭和免疫抑制方案的并发症。人们迫切需要一种替代传统器官移植的方法。使用天然或合成支架进行生物人工器官工程是一种令人振奋的新的潜在选择，可用于生成人类临床应用的功能性肺组织。天然器官支架可以通过天然组织的脱细胞生成；这些无细胞支架保留了天然器官的超微结构，并可以与自体细胞一起种植，以达到再生功能组织的目标。对于肺组织，已经采用了几种去细胞策略，然而，对于最优的方法还没有达成共识。各种类型的细胞已被研究为有效的无细胞肺支架再细胞化的潜在候选者。最有可能利用的候选细胞是那些可以容易和重复地分离、体外扩增、种植到脱细胞基质上、诱导分化为肺系细胞、并存活到功能成熟的细胞。全肺细胞悬液、内源性祖细胞、胚胎和成体干细胞以及诱导的多能干细胞(IPS)已被研究用于无细胞肺基质的再生。理想情况下，患者来源的自体细胞将用于肺再细胞化，因为它们有可能减少移植后免疫抑制的需要。一些研究已经在有限的短期功能的动物模型中进行了基本的生物工程肺支架的移植，但还需要更多的进一步研究。
For patients with end-stage lung diseases, lung transplantation is the only available therapeutic option. However, the number of suitable donor lungs is insufficient and lung transplants are complicated by significant graft failure and complications of immunosuppressive regimens. An alternative to classic organ replacement is desperately needed. Engineering of bioartificial organs using either natural or synthetic scaffolds is an exciting new potential option for generation of functional pulmonary tissue for human clinical application. Natural organ scaffolds can be generated by decellularization of native tissues; these acellular scaffolds retain the native organ ultrastructure and can be seeded with autologous cells toward the goal of regenerating functional tissues. Several decellularization strategies have been employed for lung, however, there is no consensus on the optimal approach. A variety of cell types have been investigated as potential candidates for effective recellularization of acellular lung scaffolds. Candidate cells that might be best utilized are those which can be easily and reproducibly isolated, expanded in vitro, seeded onto decellularized matrices, induced to differentiate into pulmonary lineage cells, and which survive to functional maturity. Whole lung cell suspensions, endogenous progenitor cells, embryonic and adult stem cells, and induced pluripotent stem (iPS) cells have been investigated for their applicability to repopulate acellular lung matrices. Ideally, patient-derived autologous cells would be used for lung recellularization as they have the potential to reduce the need for post-transplant immunosuppression. Several studies have performed transplantation of rudimentary bioengineered lung scaffolds in animal models with limited, short-term functionality but much further study is needed.
登录
查看更多内容
DOI：
10.1164/rccm.200811-1757st
发表时间：
2010-09-01
影响因子：
24.7
作者：
Eisner, Mark D.;Anthonisen, Nicholas;Balmes, John R.
通讯作者：
Balmes, John R.
影响因子：
4.1
作者：
Fernandes, Hugo;Mentink, Anouk;de Boer, Jan
通讯作者：
de Boer, Jan
影响因子：
3
作者：
Haykal, Siba;Soleas, John P.;Waddell, Thomas K.
通讯作者：
Waddell, Thomas K.
影响因子：
14
作者：
Daly, K. A.;Liu, S.;Badylak, S. F.
通讯作者：
Badylak, S. F.
影响因子：
14
作者：
Brown, Bryan N.;Valentin, Jolene E.;Stewart-Akers, Ann M.;McCabe, George P.;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.