Macrophage phenotype and remodeling outcomes in response to biologic scaffolds with and without a cellular component.
Macrophage phenotype and remodeling outcomes in response to biologic scaffolds with and without a cellular component.
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DOI：
10.1016/j.biomaterials.2008.11.040
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发表时间：
2009-03
期刊：
影响因子：
14
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Brown, Bryan N.;Valentin, Jolene E.;Stewart-Akers, Ann M.;McCabe, George P.;Badylak, Stephen F.
最近，巨噬细胞已被表征为具有基于受体表达、细胞因子和效应分子产生和功能的M1或M2表型。巨噬细胞表型对植入生物材料后组织重塑的影响在很大程度上尚不清楚。本研究的目的是确定植入的细胞外基质（ECM）支架内的细胞组分对巨噬细胞表型的影响，并确定巨噬细胞表型和组织重塑之间的关系。用自体体壁组织、脱细胞同种异体大鼠体壁ECM、异种猪膀胱组织或脱细胞异种猪膀胱ECM修复Sprague-Dawley大鼠腹壁肌肉组织的部分厚度缺损。在第3、7、14和28天，使用组织学、免疫组织化学和RT-PCR方法表征宿主组织反应。无细胞供试品显示主要引起M2型反应并导致结构性重塑，而含有细胞组分（甚至是自体细胞组分）的供试品主要引起M1型反应并导致致密结缔组织沉积和/或瘢痕形成。我们的结论是，ECM支架内的细胞材料的存在下，调节巨噬细胞的表型参与植入后的主机响应，并且参与主机响应的巨噬细胞的表型似乎与组织重塑的结果。
Recently, macrophages have been characterized as having an M1 or M2 phenotype based on receptor expression, cytokine and effector molecule production, and function. The effects of macrophage phenotype upon tissue remodeling following the implantation of a biomaterial are largely unknown. The objectives of this study were to determine the effects of a cellular component within an implanted extracellular matrix (ECM) scaffold upon macrophage phenotype, and to determine the relationship between macrophage phenotype and tissue remodeling. Partial-thickness defects in the abdominal wall musculature of Sprague–Dawley rats were repaired with autologous body wall tissue, acellular allogeneic rat body wall ECM, xenogeneic pig urinary bladder tissue, or acellular xenogeneic pig urinary bladder ECM. At 3, 7, 14, and 28 days the host tissue response was characterized using histologic, immunohistochemical, and RT-PCR methods. The acellular test articles were shown to elicit a predominantly M2 type response and resulted in constructive remodeling, while those containing a cellular component, even an autologous cellular component, elicited a predominantly M1 type response and resulted in deposition of dense connective tissue and/or scarring. We conclude that the presence of cellular material within an ECM scaffold modulates the phenotype of the macrophages participating in the host response following implantation, and that the phenotype of the macrophages participating in the host response appears to be related to tissue remodeling outcome.
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影响因子：
--
作者：
Gilbert, Thomas W.;Stewart-Akers, Ann M.;Woo, Savio L-Y.
通讯作者：
Woo, Savio L-Y.
影响因子：
4.4
作者：
Martinez, Fernando O.;Gordon, Siamon;Mantovani, Alberto
通讯作者：
Mantovani, Alberto
影响因子：
2.4
作者：
Badylak, S;Meurling, S;Simmons-Byrd, A
通讯作者：
Simmons-Byrd, A
影响因子：
--
作者：
McPherson, TB;Liang, H;Badylak, SF
通讯作者：
Badylak, SF
影响因子：
6.2
作者：
Parker, W;Stitzenberg, KB;Platt, JL
通讯作者：
Platt, JL