ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease.
ZYG11A Is Expressed in Epithelial Ovarian Cancer and Correlates With Low Grade Disease.
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DOI：
10.3389/fendo.2021.688104
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发表时间：
2021
影响因子：
5.2
通讯作者：
Bruchim I
中科院分区：
文献类型：
--
作者：
Achlaug L;Somri-Gannam L;Meisel-Sharon S;Sarfstein R;Dixit M;Yakar S;Hallak M;Laron Z;Werner H;Bruchim I
胰岛素样生长因子（IGF）在包括上皮性卵巢癌（EOC）在内的妇科恶性肿瘤的发生发展中起重要作用。识别有助于EOC患者诊断和评分的生物标志物在临床肿瘤学中具有根本重要性。我们最近发现ZYG11A基因是IGF1作用的新候选靶点。本研究的目的是评估ZYG11A在EOC患者中的表达，并将其表达模式与组织学分级和病理分期相关联。此外，鉴于先前的分析显示ZYG11A、p53和IGF1受体（IGF1R）之间的相互作用，我们评估了这些蛋白在EOC中的潜在协调表达。此外，在生长激素受体（GHR）敲除小鼠的卵巢和子宫中评估了BMP11a的表达。应用组织芯片技术检测36例EOC患者组织中ZYG11A、IGF1R和p53的表达。表达水平与临床参数相关。采用qPCR来评估小鼠组织中的mRNA水平。我们的分析提供了ZYG 11 A在高级别肿瘤中表达减少的证据，这与推定的肿瘤抑制作用一致。此外，注意到个体肿瘤中ZYG11A和p53水平之间的负相关性。总之，我们的数据证明了进一步探索ZYG11A作为EOC中的新型生物标志物的作用。
The insulin-like growth factors (IGF) are important players in the development of gynecological malignancies, including epithelial ovarian cancer (EOC). The identification of biomarkers that can help in the diagnosis and scoring of EOC patients is of fundamental importance in clinical oncology. We have recently identified the ZYG11A gene as a new candidate target of IGF1 action. The aim of the present study was to evaluate the expression of ZYG11A in EOC patients and to correlate its pattern of expression with histological grade and pathological stage. Furthermore, and in view of previous analyses showing an interplay between ZYG11A, p53 and the IGF1 receptor (IGF1R), we assessed a potential coordinated expression of these proteins in EOC. In addition, zyg11a expression was assessed in ovaries and uteri of growth hormone receptor (GHR) knock-out mice. Tissue microarray analysis was conducted on 36 patients with EOC and expression of ZYG11A, IGF1R and p53 was assessed by immunohistochemistry. Expression levels were correlated with clinical parameters. qPCR was employed to assess zyg11a mRNA levels in mice tissues. Our analyses provide evidence of reduced ZYG11A expression in high grade tumors, consistent with a putative tumor suppressor role. In addition, an inverse correlation between ZYG11A and p53 levels in individual tumors was noticed. Taken together, our data justify further exploration of the role of ZYG11A as a novel biomarker in EOC.
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影响因子：
5.2
作者：
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通讯作者：
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