The large-scale investigation of gene expression in Leymus chinensis stigmas provides a valuable resource for understanding the mechanisms of poaceae self-incompatibility.
The large-scale investigation of gene expression in Leymus chinensis stigmas provides a valuable resource for understanding the mechanisms of poaceae self-incompatibility.
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羊草柱头基因表达的大规模研究为了解禾本科自交不亲和机制提供了宝贵的资源
DOI：
10.1186/1471-2164-15-399
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发表时间：
2014-05-26
期刊：
影响因子：
4.4
通讯作者：
Liu G
中科院分区：
文献类型：
--
作者：
Zhou Q;Jia J;Huang X;Yan X;Cheng L;Chen S;Li X;Peng X;Liu G
研究背景：禾本科植物的配子体自交不亲和系统至少由两个独立的多等位基因位点S和Z控制。到目前为止，S和Z的基因产物是未知的。禾本科SI植物柱头区分落在其表面并支持相容性花粉管生长和渗透到柱头中的花粉粒，而识别不相容的花粉，从而抑制授粉行为。羊草Leymus chinensis（Trin.）茨维尔（绵羊草）是禾本科SI物种。通过对绵羊柱头转录组的全面分析，可以为理解花粉-柱头互作机制和牧草SI提供有价值的信息。结果：利用高通量测序技术，对绵羊成熟柱头、成熟子房和叶片的转录丰度进行了分析。这些组织的比较转录组学分析确定了1,025个特异性或优先表达的基因在羊草柱头。这些基因包含了相当大比例的基因预测功能的细胞间通讯和信号转导。共鉴定出111个转录因子基因，其中MYB、C2 H2、C3 H、FAR 1、MADS等转录因子基因的表达量最高。比较分析的羊草，水稻和拟南芥柱头特异性或优惠的数据集显示了广泛的相似性和基因本体（GO）的功能类别中的基因的比例的一些差异。在其他禾本科植物中鉴定的潜在SI候选基因也在绵羊草柱头特异性或偏好性数据集中检测到。定量实时PCR实验验证了柱头优先基因的表达模式，包括同源草SI candidate genes.Conclusions：这项研究代表了第一次大规模的调查基因表达在柱头的SI草物种。我们发现了许多可能参与花粉-柱头相互作用和SI机制的重要基因，包括编码受体样蛋白激酶（RLK）、CBL（calcineurin B-like proteins）相互作用蛋白激酶、钙依赖蛋白激酶、扩展蛋白、果胶酯酶、过氧化物酶和各种转录因子的基因。利用柱头特异或偏好基因库对L。chinensis的研究为阐明禾本科植物SI的机制提供了机会。
Background:Many Poaceae species show a gametophytic self-incompatibility (GSI) system, which is controlled by at least two independent and multiallelic loci, S and Z. Until currently, the gene products for S and Z were unknown. Grass SI plant stigmas discriminate between pollen grains that land on its surface and support compatible pollen tube growth and penetration into the stigma, whereas recognizing incompatible pollen and thus inhibiting pollination behaviors. Leymus chinensis (Trin.) Tzvel. (sheepgrass) is a Poaceae SI species. A comprehensive analysis of sheepgrass stigma transcriptome may provide valuable information for understanding the mechanism of pollen-stigma interactions and grass SI.Results:The transcript abundance profiles of mature stigmas, mature ovaries and leaves were examined using high-throughput next generation sequencing technology. A comparative transcriptomic analysis of these tissues identified 1,025 specifically or preferentially expressed genes in sheepgrass stigmas. These genes contained a significant proportion of genes predicted to function in cell-cell communication and signal transduction. We identified 111 putative transcription factors (TFs) genes and the most abundant groups were MYB, C2H2, C3H, FAR1, MADS. Comparative analysis of the sheepgrass, rice and Arabidopsis stigma-specific or preferential datasets showed broad similarities and some differences in the proportion of genes in the Gene Ontology (GO) functional categories. Potential SI candidate genes identified in other grasses were also detected in the sheepgrass stigma-specific or preferential dataset. Quantitative real-time PCR experiments validated the expression pattern of stigma preferential genes including homologous grass SI candidate genes.Conclusions:This study represents the first large-scale investigation of gene expression in the stigmas of an SI grass species. We uncovered many notable genes that are potentially involved in pollen-stigma interactions and SI mechanisms, including genes encoding receptor-like protein kinases (RLK), CBL (calcineurin B-like proteins) interacting protein kinases, calcium-dependent protein kinase, expansins, pectinesterase, peroxidases and various transcription factors. The availability of a pool of stigma-specific or preferential genes for L. chinensis offers an opportunity to elucidate the mechanisms of SI in Poaceae.
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影响因子：
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作者：
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