Effect of You-Gui-Wan on endometrial angiogenesis in controlled ovarian hyperstimulation mice model
Effect of You-Gui-Wan on endometrial angiogenesis in controlled ovarian hyperstimulation mice model
复制标题
右归丸对控制性超排卵小鼠模型子宫内膜血管生成的影响
DOI：
10.4238/gmr16039833
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发表时间：
2017
影响因子：
0.4
通讯作者：
Nan Ning
中科院分区：
文献类型：
--
作者：
Qiao zhi Yin;Ji Zhong Zhang;Li min Li;Yu lin Li;Li Huang;Nan Ning
目的：探讨右归丸改善控制性超排卵小鼠子宫内膜容受性的作用机制。方法：60只连续2个发情周期的雌性小鼠随机分为COH模型组（n = 50）和正常对照组（n = 10）。采用GnRH-a + PMSG + HCG建立COH模型，随机分为单纯模型组、益胃丸高剂量组、益胃丸中剂量组、益胃丸低剂量组和阿司匹林对照组，每组10只。给小鼠灌胃给予YGW或阿司匹林或生理盐水，每天一次，共11天。然后，分别采用H& E染色、ELISA、实时荧光定量PCR和免疫组织化学方法检测血清雌二醇（E2）和孕酮（P4）水平、子宫内膜厚度和血管数量以及血管生成因子VEGF、Ang-1、Ang-2和Tie-2在转录和翻译水平的表达。结果如下：与正常对照组相比，COH本身可增加血清E2和P4 i1/2的浓度，但降低子宫内膜血管数量和血管生成因子的蛋白表达（P< 0.05或0.01）。与单纯模型组比较，益气胃胃丸组能显著增加子宫内膜血管生成因子mRNA表达，恢复复方丹参注射液对子宫内膜血管生成的影响（P< 0.01），并能显著增加血管生成因子蛋白表达（P< 0.05或0.01）。而阿司匹林仅能恢复子宫内膜血管数目及Ang-2和Tie-2蛋白表达（P< 0.05或0.01）。结论：YGW可恢复COH对子宫内膜血管数量和血管生成因子表达的副作用，从而改善COH小鼠子宫内膜容受性
Purpose: To explore the mechanism of You-Gui-Wan (YGW) in improving endometrial receptivity in mouse model of controlled ovarian hyperstimulation (COH). Methods: Sixty female mice with two consecutive oestrous cycles were randomly divided into COH model group (n = 50) and normal control group (n = 10). The COH model was created by using GnRH-a + PMSG + HCG and further randomly divided into 5 subgroups of simple model, YGW high-dose, YGW medium-dose, YGW low-dose and aspirin control, 10 in each subgroup. YGW or aspirin or saline was administrated to the mice by gavage once a day for 11 days. Afterwards, blood and uterine samples were collected for examination of serum levels of estradiol (E2) and progesterone (P4), endometrial thickness and number of blood vessels as well as expressions of angiogenic factors of VEGF, Ang-1, Ang-2 and Tie-2 at transcription and translation levels by using H& E staining, ELISA, real-time qRT-PCR, and immunohistochemistry, respectively. Results: COH per se increased the serum concentrations of E2 and P4i¼but decreased the number of endometrial blood vessels and the protein expressions of the angiogenic factors when compared to the normal control group (P< 0.05 or 0.01). YGW increased the mRNA expressions of all angiogenic factors and restored the effect of COH on number of endometrial blood vessels (P< 0.01), and the protein expressions of the angiogenic factors when compared to the COH simple model group (P< 0.05 or 0.01). In comparison, aspirin could only restore the number of endometrial blood vessels and the protein expressions of Ang-2 and Tie-2 (P< 0.05 or 0.01). Conclusions: YGW can restore the side effect of COH on number of endometrial blood vessels and the expressions of angiogenic factors, thus improve endometrial receptivity in COH mice
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影响因子：
11.8
作者：
Gale, NW;Thurston, G;Yancopoulos, GD
通讯作者：
Yancopoulos, GD
影响因子：
3.6
作者：
Rockwell, LC;Pillai, S;Koos, RD
通讯作者：
Koos, RD
影响因子：
5.9
作者：
Nestler, G
通讯作者：
Nestler, G
影响因子：
13.3
作者：
Inhorn, Marcia C.;Patrizio, Pasquale
通讯作者：
Patrizio, Pasquale
影响因子：
0.4
作者：
Chen, X. Y.;Chen, J.;Wu, X. H.
通讯作者：
Wu, X. H.