Distribution and compartmental organization of GABAergic medium-sized spiny neurons in the mouse nucleus accumbens.
Distribution and compartmental organization of GABAergic medium-sized spiny neurons in the mouse nucleus accumbens.
复制标题
伏隔核中GABA能中型棘神经元的分布和分室组织。
DOI：
10.3389/fncir.2013.00022
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发表时间：
2013
影响因子：
3.5
通讯作者：
Valjent E
中科院分区：
文献类型：
--
作者：
Gangarossa G;Espallergues J;de Kerchove d'Exaerde A;El Mestikawy S;Gerfen CR;Hervé D;Girault JA;Valjent E
中脑核（NAc）是参与许多奖赏相关行为的关键脑区。北大西洋公约组织由核心和外壳两大部分组成，外壳又包括几个亚区。GABA能中型多刺神经元（MSN）构成NAc核和壳的输出神经元。虽然NAc核心输出的功能组织类似于背侧纹状体，但由于细胞的房室分离的复杂性，NAc壳神经元的简单分类一直难以定义。我们使用了各种BAC转基因小鼠表达增强的绿色荧光（EGFP）或Cre重组酶（Cre）的多巴胺D1，D2和D3受体和腺苷A2 a受体的启动子的控制下，解剖NAc的显微解剖。此外，使用各种免疫学标记物，我们详细描述了MSN在小鼠NAc中的分布。此外，在SKF 81297（一种D1 R样激动剂）、喹吡罗（一种D2受体（D2 R）样激动剂）、阿扑吗啡（一种非选择性DA受体激动剂）、雷氯必利（一种D2 R样拮抗剂）和精神兴奋剂药物（包括可卡因和d-苯丙胺）急性给药后，分析了NAc亚区细胞类型特异性细胞外信号调节激酶（ERK）磷酸化。每种药物在NAc中产生独特的地形和细胞类型特异性ERK激活。我们的研究结果表明存在显着差异的受体表达模式和功能激活的MSNs内的壳亚区。这项研究强调了NAc的解剖和功能异质性，这将在其进一步的研究中得到考虑。
The nucleus accumbens (NAc) is a critical brain region involved in many reward-related behaviors. The NAc comprises major compartments the core and the shell, which encompass several subterritories. GABAergic medium-sized spiny neurons (MSNs) constitute the output neurons of the NAc core and shell. While the functional organization of the NAc core outputs resembles the one described for the dorsal striatum, a simple classification of the NAc shell neurons has been difficult to define due to the complexity of the compartmental segregation of cells. We used a variety of BAC transgenic mice expressing enhanced green fluorescence (EGFP) or the Cre-recombinase (Cre) under the control of the promoter of dopamine D1, D2, and D3 receptors and of adenosine A2a receptor to dissect the microanatomy of the NAc. Moreover, using various immunological markers we characterized in detail the distribution of MSNs in the mouse NAc. In addition, cell-type specific extracellular signal-regulated kinase (ERK) phosphorylation in the NAc subterritories was analyzed following acute administration of SKF81297 (a D1R-like agonist), quinpirole (a D2 receptors (D2R)-like agonist), apomorphine (a non-selective DA receptor agonist), raclopride (a D2R-like antagonist), and psychostimulant drugs, including cocaine and d-amphetamine. Each drug generated a unique topography and cell-type specific activation of ERK in the NAc. Our results show the existence of marked differences in the receptor expression pattern and functional activation of MSNs within the shell subterritories. This study emphasizes the anatomical and functional heterogeneity of the NAc, which will have to be considered in its further study.
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影响因子：
--
作者：
Gong, Shiaoching;Yang, X William
通讯作者：
Yang, X William
DOI：
10.1016/0169-328x(92)90173-9
发表时间：
1992-07-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
FINK, JS;WEAVER, DR;REPPERT, SM
通讯作者：
REPPERT, SM
影响因子：
3.4
作者：
JONGENRELO, AL;VOORN, P;GROENEWEGEN, HJ
通讯作者：
GROENEWEGEN, HJ
DOI：
10.1523/jneurosci.3952-07.2008
发表时间：
2008-07-09
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Gerfen CR;Paletzki R;Worley P
通讯作者：
Worley P
影响因子：
2.5
作者：
Härtig, W;Riedel, A;Arendt, T
通讯作者：
Arendt, T