Quality control of photosystem II: lipid peroxidation accelerates photoinhibition under excessive illumination.
Quality control of photosystem II: lipid peroxidation accelerates photoinhibition under excessive illumination.
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DOI：
10.1371/journal.pone.0052100
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yamamoto Y
中科院分区：
文献类型：
--
作者：
Chan T;Shimizu Y;Pospíšil P;Nijo N;Fujiwara A;Taninaka Y;Ishikawa T;Hori H;Nanba D;Imai A;Morita N;Yoshioka-Nishimura M;Izumi Y;Yamamoto Y;Kobayashi H;Mizusawa N;Wada H;Yamamoto Y
环境胁迫降低了光合作用的效率，有时会对植物功能造成不可逆转的损害。当菠菜类囊体和光系统II膜在20°C或30°C下用过量可见光（100- 1，000 µmol光子m−1 s−1）照射10 min时，光系统II的最佳量子产率随着光强度和温度的升高而降低。通过在反应中心和/或附近的光化学反应产生的活性氧和内源性阳离子自由基已被牵连在D1蛋白的损伤。在这里，我们提出的证据表明，脂质过氧化诱导的照明参与损伤的D1蛋白和光系统II的捕光复合物的亚基。这是因为相当大的脂质过氧化发生在类囊体中的光，和在黑暗中添加的脂肪氧合酶外部诱导抑制光系统II活性的类囊体，单线态氧的生产，这是由电子顺磁共振自旋捕获监测，并损害D1蛋白，在平行脂质过氧化的结果。光系统II捕光复合物的亚基的丙二醛以及氧化的亚基的修饰也被观察到。我们认为，主要是单线态氧形成的脂质过氧化作用在光胁迫下参与破坏光系统II亚基。
Environmental stresses lower the efficiency of photosynthesis and sometimes cause irreversible damage to plant functions. When spinach thylakoids and Photosystem II membranes were illuminated with excessive visible light (100–1,000 µmol photons m−1 s−1) for 10 min at either 20°C or 30°C, the optimum quantum yield of Photosystem II decreased as the light intensity and temperature increased. Reactive oxygen species and endogenous cationic radicals produced through a photochemical reaction at and/or near the reaction center have been implicated in the damage to the D1 protein. Here we present evidence that lipid peroxidation induced by the illumination is involved in the damage to the D1 protein and the subunits of the light-harvesting complex of Photosystem II. This is reasoned from the results that considerable lipid peroxidation occurred in the thylakoids in the light, and that lipoxygenase externally added in the dark induced inhibition of Photosystem II activity in the thylakoids, production of singlet oxygen, which was monitored by electron paramagnetic resonance spin trapping, and damage to the D1 protein, in parallel with lipid peroxidation. Modification of the subunits of the light-harvesting complex of Photosystem II by malondialdehyde as well as oxidation of the subunits was also observed. We suggest that mainly singlet oxygen formed through lipid peroxidation under light stress participates in damaging the Photosystem II subunits.
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影响因子：
4.3
作者：
Ishikawa, Y;Nakatani, E;Yamamoto, Y
通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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