Programming protein self assembly with coiled coils
Programming protein self assembly with coiled coils
复制标题
用卷曲线圈对蛋白质自组装进行编程
DOI：
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发表时间：
2007
期刊：
影响因子：
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通讯作者：
M. Rief
中科院分区：
文献类型：
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作者：
Hendrik Dietz;T. Bornschlögl;R. Heym;Frauke König;M. Rief
控制蛋白质结构域组装成超分子结构将是在未来纳米技术应用中使用功能蛋白的重要前提。螺旋线圈是一种多聚化基序，其二聚化性质可以通过氨基酸序列来编程。在这里，我们报道了利用螺旋线圈编程的蛋白质分子的超分子自组装，并通过AFM力谱直接证明了其在单分子水平上的潜力。我们将两种不同的模型蛋白——来自人心脏titin的Ig27和绿色荧光蛋白（GFP）——通过卷曲的线圈结合伙伴连接在一起，研究了这些基本构建块自组装成线性链的能力。简单的立体约束被证明可以控制组装过程，提供了许多蛋白质可以用这种方法组装的证据。该技术的一个应用是设计具有集成力校准标准的单分子力谱多蛋白。
The controlled assembly of protein domains into supramolecular structures will be an important prerequisite for the use of functional proteins in future nanotechnology applications. Coiled coils are multimerization motifs whose dimerization properties can be programmed by amino acid sequence. Here, we report programmed supramolecular self-assembly of protein molecules using coiled coils and directly demonstrate its potential on the single molecule level by AFM force spectroscopy. We flanked two different model proteins, Ig27 from human cardiac titin and green fluorescent protein (GFP), by coiled coil binding partners and studied the capability of these elementary building blocks to self-assemble into linear chains. Simple sterical constraints are shown to control the assembly process, providing evidence that many proteins can be assembled with this method. An application for this technique is the design of polyproteins for single molecule force spectroscopy with an integrated force-calibration standard.
影响因子：
56.9
作者：
OSHEA, EK;KLEMM, JD;ALBER, T
通讯作者：
ALBER, T
影响因子：
10.8
作者：
Mathieu, F;Liao, SP;Seeman, NC
通讯作者：
Seeman, NC