Increased intraluminal release of eosinophil granule proteins EPO, ECP, EPX, and cytokines in ulcerative colitis and proctitis in segmental perfusion
Increased intraluminal release of eosinophil granule proteins EPO, ECP, EPX, and cytokines in ulcerative colitis and proctitis in segmental perfusion
复制标题
节段灌注中溃疡性结肠炎和直肠炎中嗜酸性粒细胞蛋白 EPO、ECP、EPX 和细胞因子的腔内释放增加
DOI：
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发表时间：
1999
影响因子：
9.8
通讯作者：
P. Venge
中科院分区：
文献类型：
--
作者：
M. Carlson;Y. Raab;C. Peterson;R. Hällgren;P. Venge
目的：肠粘膜嗜酸性粒细胞在炎症性肠病中的作用仍然不清楚。本研究旨在阐明溃疡性结肠炎和直肠炎患者结肠和直肠炎症病变中局部嗜酸性粒细胞活性和激活细胞因子的变化。方法：采用乙状结肠和直肠腔内节段灌注法，对18例患者（10例结肠炎和8例孤立性直肠炎）和18例健康对照进行肠道嗜酸性粒细胞活性与颗粒蛋白释放的关系研究。用放射免疫法测定灌注液中嗜酸性粒细胞颗粒蛋白：嗜酸性阳离子蛋白（ECP）、嗜酸性过氧化物酶（EPO）和嗜酸性蛋白X （EPX）的释放量。用免疫分析法分析可能的嗜酸性粒细胞启动细胞因子、粒细胞/巨噬细胞集落刺激因子（GM-CSF）和白细胞介素8 （IL-8）的腔内释放。结果：与对照组相比，结肠炎和直肠炎患者的ECP、EPO和EPX的粘膜释放增加了10- 20倍。在结肠炎和直肠炎患者中，GM-CSF和IL-8的腔内释放数倍增强。我们还发现所有三种嗜酸性粒细胞颗粒蛋白与结肠炎患者乙状结肠节段IL-8/GM-CSF水平之间存在相关性。结论：我们得出结论，ECP、EPO和EPX向结肠灌注液释放的增加表明嗜酸性粒细胞参与结肠炎和直肠炎患者的局部疾病。IL-8和GM-CSF可能在结肠炎的嗜酸性粒细胞积累和启动中起作用。
OBJECTIVES:The role of the eosinophil granulocyte in bowel mucosa in inflammatory bowel disease still remains obscure. The present study was performed in order to elucidate the local eosinophil activity and activating cytokines in the inflamed lesions of colon and rectum in patients with ulcerative colitis and proctitis.METHODS:The activity of intestinal eosinophils with respect to the release of granule proteins was studied in 18 patients (10 with colitis and 8 with isolated proctitis) and 18 healthy controls, using intraluminal segmental perfusion of the sigmoid colon and rectum. The released amounts of eosinophil granule proteins: eosinophil cationic protein (ECP), eosinophil peroxidase (EPO), and eosinophil protein X (EPX) to perfusion fluid were determined by radioimmunoassays. The intraluminal release of possible eosinophil priming cytokines granulocyte/macrophage-colony stimulating factor (GM-CSF) and interleukin 8 (IL-8), were analyzed by immunoassays.RESULTS:The mucosal release of ECP, EPO, and EPX was increased 10- to 20-fold in patients with colitis and proctitis compared with controls. The intraluminal release of GM-CSF and IL-8, was several-fold enhanced in patients with colitis and proctitis. We also found a correlation between all three eosinophil granule proteins and the levels of IL-8/GM-CSF in the sigmoidal segments of patients with colitis.CONCLUSIONS:We conclude that the increased release of ECP, EPO, and EPX to colorectal perfusion fluid indicate eosinophil involvement in the local disease in patients with colitis and proctitis. IL-8 and GM-CSF may play a role in eosinophil accumulation and priming in colitis.
嗜酸性粒细胞衍生的神经毒素和人肝核糖核酸酶。
DOI：
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发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Sorrentino,S;Glitz,DG;Hamann,KJ;Loegering,DA;Checkel,JL;Gleich,GJ
通讯作者：
Gleich,GJ
影响因子：
14.2
作者：
GLEICH, GJ;FLAVAHAN, NA;VANHOUTTE, PM
通讯作者：
VANHOUTTE, PM
激活的人类嗜酸性粒细胞。
影响因子：
2.8
作者：
Melo,RossanaCN;Weller,PeterF;Dvorak,AnnM
通讯作者：
Dvorak,AnnM