Salivary metabolite profiling distinguishes patients with oral cavity squamous cell carcinoma from normal controls.
Salivary metabolite profiling distinguishes patients with oral cavity squamous cell carcinoma from normal controls.
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DOI：
10.1371/journal.pone.0204249
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Chen C
中科院分区：
文献类型：
--
作者：
Lohavanichbutr P;Zhang Y;Wang P;Gu H;Nagana Gowda GA;Djukovic D;Buas MF;Raftery D;Chen C
口腔鳞状细胞癌（OCC）和口咽鳞状细胞癌（OPC）是世界上最常见的癌症之一，具有高死亡率和高发病率。本研究的目的是寻找潜在的生物标志物来区分OCC/OPC与正常对照，并区分有和没有淋巴结转移的OCC患者。我们使用四种分析平台（NMR， LC-MS/MS靶向水样，LC-Q-TOF全局水样和全局脂质组学）检测了101例OCC、58例OPC和35例正常对照的唾液样本。OCC和正常对照的样本分为发现组和验证组。使用线性回归调整年龄、性别、种族和实验批次，我们发现两种代谢物（甘氨酸和脯氨酸）的水平在OCC和对照组之间有显著差异（发现组和验证组的FDR均< 0.1），但在OPC和对照组之间或在有和没有淋巴结转移的OCC之间，代谢物水平没有明显差异。四种代谢物，包括甘氨酸、脯氨酸、瓜氨酸和鸟氨酸，在发现和验证集中都与早期OCC相关。需要进一步的研究来证实这些结果，以发展唾液代谢物作为诊断标志物。
Oral cavity squamous cell carcinoma (OCC) and oropharyngeal squamous cell carcinoma (OPC) are among the most common cancers worldwide and are associated with high mortality and morbidity. The purpose of this study is to identify potential biomarkers to distinguish OCC/OPC from normal controls and to distinguish OCC patients with and without nodal metastasis. We tested saliva samples from 101 OCC, 58 OPC, and 35 normal controls using four analytical platforms (NMR, targeted aqueous by LC-MS/MS, global aqueous and global lipidomics by LC-Q-TOF). Samples from OCC and normal controls were divided into discovery and validation sets. Using linear regression adjusting for age, sex, race and experimental batches, we found the levels of two metabolites (glycine and proline) to be significantly different between OCC and controls (FDR < 0.1 for both discovery and validation sets) but did not find any appreciable differences in metabolite levels between OPC and controls or between OCC with and without nodal metastasis. Four metabolites, including glycine, proline, citrulline, and ornithine were associated with early stage OCC in both discovery and validation sets. Further study is warranted to confirm these results in the development of salivary metabolites as diagnostic markers.
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影响因子：
50.3
作者：
Carroll PA;Diolaiti D;McFerrin L;Gu H;Djukovic D;Du J;Cheng PF;Anderson S;Ulrich M;Hurley JB;Raftery D;Ayer DE;Eisenman RN
通讯作者：
Eisenman RN
影响因子：
64.8
作者：
通讯作者：
--
研究代谢与蛋白质组生物量之间平衡的定量方法：从甘氨酸开始
影响因子：
16.6
作者：
Gu, Haiwei;Carroll, Patrick A.;Raftery, Daniel
通讯作者：
Raftery, Daniel
影响因子：
46.9
作者：
Fiehn, O;Kopka, J;Willmitzer, L
通讯作者：
Willmitzer, L
影响因子：
21.1
作者：
Anderson NM;Mucka P;Kern JG;Feng H
通讯作者：
Feng H