Structure-Guided Reprogramming of a Hydroxylase To Halogenate Its Small Molecule Substrate.
Structure-Guided Reprogramming of a Hydroxylase To Halogenate Its Small Molecule Substrate.
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DOI：
10.1021/acs.biochem.6b01173
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发表时间：
2017-01-24
期刊：
影响因子：
2.9
通讯作者：
Boal AK
中科院分区：
文献类型：
--
作者：
Mitchell AJ;Dunham NP;Bergman JA;Wang B;Zhu Q;Chang WC;Liu X;Boal AK
Enzymatic installation of chlorine/bromine upon unactivated carbon centers provides a versatile, selective, and environmentally-friendly alternative to chemical halogenation. Iron(II)- and 2-(oxo)-glutarate (FeII/2OG)-dependent halogenases are powerful biocatalysts that are capable of cleaving aliphatic C-H bonds to install useful functional groups, including halogens. Using the structure of the Fe/2OG halogenase, WelO5, in complex with its small-molecule substrate, we identified a similar N-acyl amino acid hydroxylase, SadA, and reprogrammed it to halogenate its substrate, thereby generating a new chiral haloalkyl center. The work high-lights the potential of FeII/2OG enzymes as platforms for development of novel stereospecific catalysts for late-stage C-H functionalization.
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影响因子：
2.9
作者：
Jiang, Wei;Heemstra, John R., Jr.;Forseth, Ry R.;Neumann, Christopher S.;Manaviazar, Soraya;Schroeder, Frank C.;Hale, Karl J.;Walsh, Christopher T.
通讯作者：
Walsh, Christopher T.
DOI：
10.1073/pnas.0909649106
发表时间：
2009-10-20
影响因子：
11.1
作者：
Matthews, Megan L.;Neumann, Christopher S.;Bollinger, J. Martin, Jr.
通讯作者：
Bollinger, J. Martin, Jr.
影响因子：
3.2
作者：
Hillwig, Matthew L.;Fuhrman, Heather A.;Ittiamornkul, Kuljira;Sevco, Tyler J.;Kwak, Daniel H.;Liu, Xinyu
通讯作者：
Liu, Xinyu
影响因子：
14.8
作者：
Hillwig, Matthew L.;Liu, Xinyu
通讯作者：
Liu, Xinyu
影响因子：
3.9
作者：
Grzyska, Piotr K.;Muller, Tina A.;Hausinger, Robert P.
通讯作者：
Hausinger, Robert P.