Reaction of human albumin with aspirin in vitro: mass spectrometric identification of acetylated lysines 199, 402, 519, and 545.
Reaction of human albumin with aspirin in vitro: mass spectrometric identification of acetylated lysines 199, 402, 519, and 545.
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DOI：
10.1016/j.bcp.2009.10.007
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发表时间：
2010-03-01
影响因子：
5.8
通讯作者：
Lockridge, Oksana
中科院分区：
文献类型：
--
作者：
Liyasova, Mariya S.;Schopfer, Lawrence M.;Lockridge, Oksana
人血浆中的阿司匹林酯酶活性由丁酰胆碱酯酶和白蛋白引起。我们的目标是确定氨基酸残基参与阿司匹林酯酶活性的白蛋白。用阿司匹林处理游离脂肪酸人血白蛋白和人血浆5 min至24 h。通过胰蛋白酶肽的LC/MS/MS和MALDI-TOF/TOF质谱鉴定乙酰化残基。用0.3 mM阿司匹林处理导致Lys-199、Lys-402、Lys-519和Lys-545的乙酰化。用20 mM阿司匹林处理导致26个赖氨酸的乙酰化。在任何条件下，Tyr-411均未发生乙酰化。乙酰化赖氨酸在pH 7.4、37 °C下稳定至少21天。如酯酶活性染色凝胶所示，阿司匹林乙酰化的白蛋白与β-乙酸萘酯的酯酶活性降低。结论：白蛋白的阿司匹林酯酶活性是一种伪酯酶活性，其中阿司匹林稳定地乙酰化赖氨酸并释放水杨酸盐。
The aspirin esterase activity of human plasma is due to butyrylcholinesterase and albumin. Our goal was to identify the amino acid residues involved in the aspirin esterase activity of albumin. Fatty acid free human albumin and human plasma were treated with aspirin for 5 min to 24 h. Acetylated residues were identified by LC/MS/MS and MALDI-TOF/TOF mass spectrometry of tryptic peptides. Treatment with 0.3 mM aspirin resulted in acetylation of Lys-199, Lys-402, Lys-519, and Lys-545. Treatment with 20 mM aspirin resulted in acetylation of 26 lysines. There was no acetylation of Tyr-411, under any conditions. Acetylated lysine was stable for at least 21 days at pH 7.4, 37 °C. Albumin acetylated by aspirin had reduced esterase activity with beta-naphthyl acetate as shown on gels stained for esterase activity. It was concluded that the aspirin esterase activity of albumin is a pseudo-esterase activity in which aspirin stably acetylates lysines and releases salicylate.
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DOI：
10.1254/jjp.29.581
发表时间：
1979-01-01
期刊：
JAPANESE JOURNAL OF PHARMACOLOGY
影响因子：
--
作者：
MORIKAWA, M;INOUE, M;SUGIURA, M
通讯作者：
SUGIURA, M
影响因子：
7.3
作者：
Díaz, N;Suárez, D;Merz, KM
通讯作者：
Merz, KM
影响因子：
7.4
作者：
Trelle, Morten B.;Jensen, Ole N.
通讯作者：
Jensen, Ole N.
影响因子：
3.8
作者：
BAKAR, SK;NIAZI, S
通讯作者：
NIAZI, S
影响因子：
3.8
作者：
ROWLAND, M;SHOLKOFF, SD;RIEGELMAN, S
通讯作者：
RIEGELMAN, S