Andrology: Analysis of chromosome constitution of human spermatozoa with normal and aberrant head morphologies after injection into mouse oocytes
Andrology: Analysis of chromosome constitution of human spermatozoa with normal and aberrant head morphologies after injection into mouse oocytes
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男科学：头部形态正常和异常的人类精子注射到小鼠卵母细胞后的染色体组成分析
DOI：
10.1093/oxfordjournals.humrep.a019521
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发表时间：
1996
影响因子：
6.1
通讯作者：
Ryuzo Yanagimachi
中科院分区：
文献类型：
--
作者：
J. D. Lee;Y. Kamiguchi;Ryuzo Yanagimachi
将单个精子注入小鼠卵母细胞后，测定了人类精子的染色体组成。在279个第一卵裂中期停滞的卵子中，有200个（71.7%）适合精子染色体分析。在头部形态正常的精子中，染色体数目和结构畸变的精子发生率分别为1.3%和6.9%，与先前使用仓鼠卵母细胞-人精子融合系统的研究中发现的值相当。携带X染色体的精子与携带Y染色体的精子的比例与预期的1：1比例没有显著差异。头部无定形、圆形和细长的精子染色体结构畸变的发生率约为头部形态正常的精子的4倍（26.1%），而非整倍体的发生率在两组之间没有显著差异。头部较大的精子染色体畸变未见增加。头部较小的精子也是如此。虽然本研究所用的样本量很小，但结果仍然表明精子头部的某些形态异常与其染色体缺陷有关。
The chromosome constitution of human spermatozoa was determined after injecting individual spermatozoa into mouse oocytes. Of a total 279 eggs arrested at first cleavage metaphase, 200 (71.7%) were suitable for the analysis of sperm chromosomes. Incidences of spermatozoa with numerical and structural chromosome aberrations were 1.3 and 6.9% respectively in spermatozoa with normal head morphology, showing values comparable with those found in previous studies using the hamster oocyte-human sperm fusion system. The ratio of X- to Y-bearing spermatozoa did not differ significantly from the expected 1:1 ratio. The incidence of structural chromosome aberrations was about four times higher in spermatozoa with amorphous, round and elongated heads (26.1%) than in those with morphologically normal heads, whereas the incidence of aneuploidy was not significantly different between the two groups. No increase in chromosome aberrations was found in spermatozoa with large heads. The same was true for spermatozoa with small heads. Although the sample size used in this study is rather small, the results nevertheless indicate that some morphological abnormalities in the sperm heads are associated with their chromosome defects.
影响因子：
3.6
作者：
CHATOT, CL;LEWIS, JL;ZIOMEK, CA
通讯作者：
ZIOMEK, CA
DOI：
10.1530/jrf.0.0860679
发表时间：
1989-07-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
CHATOT, CL;ZIOMEK, CA;TORRES, I
通讯作者：
TORRES, I