Effects of long-term oral administration of arachidonic acid and docosahexaenoic acid on the immune functions of young rats.
Effects of long-term oral administration of arachidonic acid and docosahexaenoic acid on the immune functions of young rats.
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DOI：
10.3390/nu5061949
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发表时间：
2013-05-29
期刊：
影响因子：
5.9
通讯作者：
Shido O
中科院分区：
文献类型：
--
作者：
Juman S;Hashimoto M;Katakura M;Inoue T;Tanabe Y;Arita M;Miki T;Shido O
自然杀伤（NK）细胞具有许多功能活性，包括细胞毒性和产生细胞因子和趋化因子的能力。NK细胞活性部分由类二十烷酸调节，类二十烷酸由花生四烯酸（ARA）和二十碳五烯酸（EPA）产生。在这项研究中，我们研究了ARA或二十二碳六烯酸（DHA）的长期治疗对年轻大鼠NK细胞的细胞毒性作用的影响，这些大鼠用非鱼油饮食喂养了两代。对照油、ARA（240 mg/kg BW/天）或DHA（240 mg/kg BW/天）经口给予大鼠13周，然后测定脾脏NK细胞对YAC-1小鼠淋巴瘤细胞系的细胞毒性活性，以及类二十二烷酸或类二十烷酸和炎性细胞因子的血浆水平。长期ARA给药显著抑制NK细胞的细胞毒活性。此外，ARA给药显著增加了ARA、前列腺素（PG）E2和PGD 2的血浆水平。然而，与对照组大鼠相比，DHA给药没有产生任何不同的效果。此外，炎性细胞因子水平不受ARA或DHA给药的影响。这些结果表明，长期给予ARA对大鼠脾淋巴细胞中NK细胞的肿瘤细胞毒性具有抑制作用，这是由于年轻大鼠血浆ARA水平升高，ARA合成PGE 2和PGD 2的能力增强所致。
Natural killer (NK) cells have many functional activities, including cytotoxicity and the capacity to produce cytokines and chemokines. NK cell activity is regulated partly by eicosanoids, which are produced from arachidonic acid (ARA) and eicosapentaenoic (EPA) acid. In this study, we investigated the effects of long-term therapy with ARA or docosahexaenoic acid (DHA) on the cytotoxic effects of the NK cells of young rats, which were fed on a nonfish oil diet for two generations. Control oil, ARA (240 mg/kg BW/day) or DHA (240 mg/kg BW/day) were orally administrated to the rats for 13 weeks before determining the cytotoxic activity of NK cells from the spleen against YAC-1 mouse lymphoma cell line, as well as the plasma levels of docosanoids or eicosanoids and inflammatory cytokines. Long-term ARA administration significantly suppressed the cytotoxic activity of NK cells. Moreover, ARA administration significantly increased the plasma levels of ARA, prostaglandin (PG) E2, and PGD2. However, DHA administration did not produce any different effects compared with those in the control rats. Furthermore, the inflammatory cytokine levels were not affected by the administration of ARA or DHA. These results suggest that long-term ARA administration has an inhibitory effect on the tumor cytotoxicity of NK cells in rat spleen lymphocytes owing to the enhanced synthesis of PGE2 and PGD2 from ARA because of the elevated plasma ARA levels in young rats.
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影响因子：
4.4
作者：
Chen, Yingying;Perussia, Bice;Campbell, Kerry S.
通讯作者：
Campbell, Kerry S.
影响因子：
20.3
作者：
Monneret, G;Gravel, S;Powell, WS
通讯作者：
Powell, WS
影响因子：
4.4
作者：
Lu, Ling-Min;Zavitz, Caleb C. J.;Stampfli, Martin R.
通讯作者：
Stampfli, Martin R.
影响因子：
3.9
作者：
BROUARD, C;PASCAUD, M
通讯作者：
PASCAUD, M
DOI：
10.1084/jem.193.2.255
发表时间：
2001-01-15
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hirai H;Tanaka K;Yoshie O;Ogawa K;Kenmotsu K;Takamori Y;Ichimasa M;Sugamura K;Nakamura M;Takano S;Nagata K
通讯作者：
Nagata K