Arecoline Induces an Excitatory Response in Ventral Tegmental Area Dopaminergic Neurons in Anesthetized Rats.
Arecoline Induces an Excitatory Response in Ventral Tegmental Area Dopaminergic Neurons in Anesthetized Rats.
复制标题
槟榔碱诱导麻醉大鼠腹侧被盖区多巴胺能神经元的兴奋反应
DOI：
10.3389/fphar.2022.872212
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发表时间：
2022
影响因子：
5.6
通讯作者：
Zhang, Changzheng
中科院分区：
文献类型：
--
作者：
Lan, Qinghui;Guan, Peiqing;Huang, Chunzheng;Huang, Shile;Zhou, Peiling;Zhang, Changzheng
关键词：
槟榔碱是槟榔中主要的精神活性生物碱。槟榔是槟榔科植物Areca catechu L.的咀嚼种子，是生长在热带、亚热带国家的流行植物，长期使用会产生依赖性。然而，这种依赖背后的机制在很大程度上仍不清楚，因此，尚未制定有效的干预措施来停止这种依赖。本研究旨在探讨槟榔碱对腹侧被盖区（VTA）神经元的影响。大鼠麻醉并开颅后，将电生理电极放入 VTA 中以获得细胞外记录。然后计算并分析槟榔碱治疗前后多巴胺能和GABA能神经元的平均放电率。还分析了多巴胺能神经元的爆发特征。结果表明，槟榔碱能显着提高多巴胺能神经元的放电率，但不能显着提高 GABA 能神经元的放电率。此外，槟榔碱在多巴胺能神经元中引起显着的爆发放电，包括爆发速率的增加、爆发持续时间的延长以及每次爆发的尖峰数量的增加。总的来说，研究结果表明，槟榔碱显着兴奋 VTA 多巴胺能神经元，这可能是槟榔依赖的潜在机制，也是槟榔戒断治疗的潜在目标。
Arecoline is the principle psychoactive alkaloid in areca nuts. Areca nuts are chewable seeds of Areca catechu L., which are epidemic plants that grow in tropical and subtropical countries and cause dependency after long-term use. However, the mechanisms underlying such dependency remain largely unclear, and therefore, no effective interventions for its cessation have been developed. The present study aimed to examine the effects of arecoline on neurons of the ventral tegmental area (VTA). After rats were anesthetized and craniotomized, electrophysiological electrodes were lowered into the VTA to obtain extracellular recordings. The mean firing rate of dopaminergic and GABAergic neurons were then calculated and analyzed before and after arecoline treatment. The burst characteristics of the dopaminergic neurons were also analyzed. The results showed that arecoline evoked a significant enhancement of the firing rate of dopaminergic neurons, but not GABAergic neurons. Moreover, arecoline evoked remarkable burst firings in the dopaminergic neurons, including an increase in the burst rate, elongation in the burst duration, and an enhancement in the number of spikes per burst. Collectively, the findings revealed that arecoline significantly excited VTA dopaminergic neurons, which may be a mechanism underlying areca nut dependency and a potential target for areca nut cessation therapy.
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影响因子：
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作者：
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DOI：
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