Origin of gene overlap: the case of TCP1 and ACAT2.
Origin of gene overlap: the case of TCP1 and ACAT2.
复制标题
基因重叠的起源：TCP1 和 ACAT2 的情况。
作者：
Seikou Shintani;C. O’hUigin;Satoru Toyosawa;Věra Michalová;Jan Klein
人类乙酰-辅酶A乙酰转移酶2基因ACAT2编码硫解酶，这是一种参与脂肪代谢的酶。人类T-复合体蛋白1基因TCP1编码伴侣蛋白家族的一个分子伴侣。这两个基因通过其3‘-非翻译区重叠，它们的编码序列以尾巴到尾巴的方向位于相反的DNA链上。为了找出这种重叠是如何在进化中出现的，我们鉴定了斑马鱼、非洲蟾蜍、凯门鳄、鸭嘴兽、负鼠和袋鼠的同源基因。在每个物种中，使用标准或长时间的聚合酶链式反应来确定ACAT2和TCP1同源物是否紧密相连，如果是，则确定它们是否重叠。结果显示，这种重叠显然是在从治疗爬行动物向哺乳动物的转变过程中出现的，并且已经保留了2亿年。部分重叠的非翻译区显示出显著的序列保守性。这种重叠可能是在染色体重排过程中出现的，该重排将这两个不相关且之前分离的基因聚集在一起。转座基因偶然发现的转座基因中的一个或两个，这些信号是处理它们的转录本所必需的，它们出现在伴侣基因的非编码链上
The human acetyl-CoA acetyltransferase 2 gene, ACAT2, codes for a thiolase, an enzyme involved in lipid metabolism. The human T-complex protein 1 gene, TCP1, encodes a molecular chaperone of the chaperonin family. The two genes overlap by their 3'-untranslated regions, their coding sequences being located on opposite DNA strands in a tail-to-tail orientation. To find out how the overlap might have arisen in evolution, the homologous genes of the zebrafish, the African toad, caiman, platypus, opossum, and wallaby were identified. In each species, standard or long polymerase chain reactions were used to determine whether the ACAT2 and TCP1 homologs are closely linked and, if so, whether they overlap. The results reveal that the overlap apparently arose during the transition from therapsid reptiles to mammals and has been retained for >200 million years. Part of the overlapping untranslated region shows remarkable sequence conservation. The overlap presumably arose during the chromosomal rearrangement that brought the two unrelated and previously separated genes together. One or both of the transposed genes found by chance signals that are necessary for the processing of their transcripts to be present on the noncoding strand of the partner gene.
大鼠线粒体细胞色素 P-450c27/25 和生长激素诱导型丝氨酸蛋白酶抑制剂的 mRNA 5 末端区域之间的序列互补性。
DOI：
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发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Shayiq,RM;Avadhani,NG
通讯作者：
Avadhani,NG
PCNA mRNA 具有黄新月体 RNA 的 3UTR 反义，并且定位于海鞘卵和胚胎中。
DOI：
10.1006/dbio.1996.0195
发表时间：
1996
期刊：
Developmental biology.
影响因子：
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作者：
Swalla,BJ;Jeffery,WR
通讯作者：
Jeffery,WR