Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms.
Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organisms.
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DOI：
10.1016/j.xpro.2021.100486
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发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
McKnight JN
中科院分区：
文献类型：
--
作者：
McKnight LE;Crandall JG;Bailey TB;Banks OGB;Orlandi KN;Truong VN;Donovan DA;Waddell GL;Wiles ET;Hansen SD;Selker EU;McKnight JN
微球菌核酸酶测序技术(MNase-seq)用于定位核小体在真核基因组中的位置，以研究染色质结构与DNA依赖过程的关系。目前的方案需要至少两天的时间来分离受核小体保护的DNA片段。我们已经为酿酒酵母和其他真菌开发了一种简化的程序，只需要三个小时。改进的方案被开发用于野生真菌和哺乳动物细胞。这种从几种生物中快速产生可用于测序的核小体足迹的方法使MNase-SEQ更快、更容易，并且减少了化学废物。一种快速制备微球菌核小体核小体足迹的方法，省去了苯酚和氯仿的使用，并减少了适应多种生物的细胞数量。利用MNase-SEQ定位真核基因组中的核小体位置，以研究染色质结构与DNA依赖过程之间的关系。目前的方案需要至少两天的时间来分离受核小体保护的DNA片段。我们已经为酿酒酵母和其他真菌开发了一种简化的程序，只需要三个小时。改进的方案被开发用于野生真菌和哺乳动物细胞。这种从几种生物中快速产生可用于测序的核小体足迹的方法使MNase-SEQ更快、更容易，并且减少了化学废物。
MNase-seq (micrococcal nuclease sequencing) is used to map nucleosome positions in eukaryotic genomes to study the relationship between chromatin structure and DNA-dependent processes. Current protocols require at least two days to isolate nucleosome-protected DNA fragments. We have developed a streamlined protocol for S. cerevisiae and other fungi which takes only three hours. Modified protocols were developed for wild fungi and mammalian cells. This method for rapidly producing sequencing-ready nucleosome footprints from several organisms makes MNase-seq faster and easier, with less chemical waste. A fast way to prepare micrococcal nuclease nucleosome footprints for MNase-seq Eliminates use of phenol and chloroform and reduces the amount of cells required Adaptable for a variety of organisms MNase-seq is used to map nucleosome positions in eukaryotic genomes to study the relationship between chromatin structure and DNA-dependent processes. Current protocols require at least two days to isolate nucleosome-protected DNA fragments. We have developed a streamlined protocol for S. cerevisiae and other fungi which takes only three hours. Modified protocols were developed for wild fungi and mammalian cells. This method for rapidly producing sequencing-ready nucleosome footprints from several organisms makes MNase-seq faster and easier, with less chemical waste.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
7
作者：
McKnight JN;Tsukiyama T;Bowman GD
通讯作者：
Bowman GD
影响因子：
3.3
作者：
Klocko, Andrew D.;Uesaka, Miki;Selker, Eric U.
通讯作者：
Selker, Eric U.
DOI：
10.1083/jcb.200604072
发表时间：
2006-07-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Allen C;Büttner S;Aragon AD;Thomas JA;Meirelles O;Jaetao JE;Benn D;Ruby SW;Veenhuis M;Madeo F;Werner-Washburne M
通讯作者：
Werner-Washburne M
影响因子：
16
作者：
McKnight, Jeffrey N.;Boerma, Joseph W.;Tsukiyama, Toshio
通讯作者：
Tsukiyama, Toshio