Isolation of quiescent and nonquiescent cells from yeast stationary-phase cultures.
Isolation of quiescent and nonquiescent cells from yeast stationary-phase cultures.
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DOI：
10.1083/jcb.200604072
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发表时间：
2006-07-03
期刊：
影响因子：
--
通讯作者：
Werner-Washburne M
中科院分区：
文献类型：
--
作者：
Allen C;Büttner S;Aragon AD;Thomas JA;Meirelles O;Jaetao JE;Benn D;Ruby SW;Veenhuis M;Madeo F;Werner-Washburne M
静止是最常见的，也是最不了解的细胞周期状态。这部分是因为静止细胞的纯群体通常难以分离。我们报告的静态和非静态的细胞从静止相（SP）酵母培养物的密度梯度离心分离和表征。静止细胞是葡萄糖耗尽后形成的致密的、未出芽的子细胞。它们同时重新进入有丝分裂细胞周期，表明它们处于G0状态。非静止细胞密度较低，异质性较强，由复制年龄较大的异步细胞组成，这些细胞迅速失去繁殖能力。显微镜和流式细胞仪分析显示，non静止细胞积累更多的活性氧比静止细胞，超过21天，约一半的细胞凋亡和坏死的迹象。从SP培养物中分离静止和非静止酵母细胞的能力为静止、时序和复制性衰老、细胞凋亡和细胞周期的研究提供了一种新颖的、易处理的实验系统。
Quiescence is the most common and, arguably, most poorly understood cell cycle state. This is in part because pure populations of quiescent cells are typically difficult to isolate. We report the isolation and characterization of quiescent and nonquiescent cells from stationary-phase (SP) yeast cultures by density-gradient centrifugation. Quiescent cells are dense, unbudded daughter cells formed after glucose exhaustion. They synchronously reenter the mitotic cell cycle, suggesting that they are in a G0 state. Nonquiescent cells are less dense, heterogeneous, and composed of replicatively older, asynchronous cells that rapidly lose the ability to reproduce. Microscopic and flow cytometric analysis revealed that nonquiescent cells accumulate more reactive oxygen species than quiescent cells, and over 21 d, about half exhibit signs of apoptosis and necrosis. The ability to isolate both quiescent and nonquiescent yeast cells from SP cultures provides a novel, tractable experimental system for studies of quiescence, chronological and replicative aging, apoptosis, and the cell cycle.
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影响因子：
12.3
作者：
Aragon AD;Quiñones GA;Thomas EV;Roy S;Werner-Washburne M
通讯作者：
Werner-Washburne M
影响因子：
9.8
作者：
Coller HA;Sang L;Roberts JM
通讯作者：
Roberts JM
DOI：
10.1007/s004380050017
发表时间：
1999-06-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Charizanis, C;Juhnke, H;Entian, KD
通讯作者：
Entian, KD
影响因子：
4.1
作者：
Bonini, BM;van Vaeck, C;Thevelein, JM
通讯作者：
Thevelein, JM
DOI：
10.1016/s0304-4165(01)00134-9
发表时间：
2001-07-02
影响因子：
3
作者：
Lee, JS;Huh, WK;Kang, SO
通讯作者：
Kang, SO