Defective DNA repair and increased genomic instability in Artemis-deficient murine cells.
Defective DNA repair and increased genomic instability in Artemis-deficient murine cells.
复制标题
缺陷的DNA修复和增加的缺乏小鼠细胞中的基因组不稳定性。
DOI：
10.1084/jem.20021891
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发表时间：
2003-03-03
影响因子：
15.3
通讯作者：
Sekiguchi, J
中科院分区：
文献类型：
--
作者：
Rooney, S;Alt, FW;Lombard, D;Whitlow, S;Eckersdorff, M;Fleming, J;Fugmann, S;Ferguson, DO;Schatz, DG;Sekiguchi, J
In developing lymphocytes, the recombination activating gene endonuclease cleaves DNA between V, D, or J coding and recombination signal (RS) sequences to form hairpin coding and blunt RS ends, which are fused to form coding and RS joins. Nonhomologous end joining (NHEJ) factors repair DNA double strand breaks including those induced during VDJ recombination. Human radiosensitive severe combined immunodeficiency results from lack of Artemis function, an NHEJ factor with in vitro endonuclease/exonuclease activities. We inactivated Artemis in murine embryonic stem (ES) cells by targeted mutation. Artemis deficiency results in impaired VDJ coding, but not RS, end joining. In addition, Artemis-deficient ES cells are sensitive to a radiomimetic drug, but less sensitive to ionizing radiation. VDJ coding joins from Artemis-deficient ES cells, which surprisingly are distinct from the highly deleted joins consistently obtained from DNA-dependent protein kinase catalytic subunit–deficient ES cells, frequently lack deletions and often display large junctional palindromes, consistent with a hairpin coding end opening defect. Strikingly, Artemis-deficient ES cells have increased chromosomal instability including telomeric fusions. Thus, Artemis appears to be required for a subset of NHEJ reactions that require end processing. Moreover, Artemis functions as a genomic caretaker, most notably in prevention of translocations and telomeric fusions. As Artemis deficiency is compatible with human life, Artemis may also suppress genomic instability in humans.
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影响因子：
15.3
作者：
Ferrier, P;Covey, L R;Li, S C;Suh, H;Malynn, B A;Blackwell, T K;Morrow, M A;Alt, F W
通讯作者：
Alt, F W
DOI：
10.1084/jem.20020851
发表时间：
2002-08-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Difilippantonio MJ;Petersen S;Chen HT;Johnson R;Jasin M;Kanaar R;Ried T;Nussenzweig A
通讯作者：
Nussenzweig A
影响因子：
16
作者：
Besmer, E;Mansilla-Soto, J;Cortes, P
通讯作者：
Cortes, P
影响因子：
11.4
作者：
BLACKWELL, TK;MALYNN, BA;ALT, FW
通讯作者：
ALT, FW
影响因子：
10.5
作者：
CARROLL, AM;BOSMA, MJ
通讯作者：
BOSMA, MJ