Human umbilical cord mesenchymal stem cell-loaded amniotic membrane for the repair of radial nerve injury.
Human umbilical cord mesenchymal stem cell-loaded amniotic membrane for the repair of radial nerve injury.
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DOI：
10.3969/j.issn.1673-5374.2013.36.010
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发表时间：
2013-12-25
影响因子：
6.1
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Li Z;Qin H;Feng Z;Liu W;Zhou Y;Yang L;Zhao W;Li Y
在这项研究中，我们将人脐带间充质干细胞负载到具有上皮细胞的人羊膜上以制备神经导管，即，一个相对封闭的神经再生室神经松解后，将制备好的神经导管与神经外膜缝合固定，细胞位于导管内表面。同时将1.0 mL人脐带间充质干细胞悬液以1.0 cm间隔注入损伤的桡神经远端和近端。将总计1.75 × 107个细胞接种在羊膜上。对照组仅行神经松解术。在细胞移植后12周，超过80%的患者在肌肉力量、触觉和疼痛感觉方面表现出明显的改善。相比之下，仅在55-65%的对照患者中观察到这些改善。术后8周和12周，移植组桡神经支配区的肌电生理功能明显优于对照组。细胞移植后，未观察到免疫排斥反应。这些发现表明负载人脐带间充质干细胞的羊膜可用于修复桡神经损伤。
In this study, we loaded human umbilical cord mesenchymal stem cells onto human amniotic membrane with epithelial cells to prepare nerve conduits, i.e., a relatively closed nerve regeneration chamber. After neurolysis, the injured radial nerve was enwrapped with the prepared nerve conduit, which was fixed to the epineurium by sutures, with the cell on the inner surface of the conduit. Simultaneously, a 1.0 mL aliquot of human umbilical cord mesenchymal stem cell suspension was injected into the distal and proximal ends of the injured radial nerve with 1.0 cm intervals. A total of 1.75 × 107 cells were seeded on the amniotic membrane. In the control group, patients received only neurolysis. At 12 weeks after cell transplantation, more than 80% of patients exhibited obvious improvements in muscular strength, and touch and pain sensations. In contrast, these improvements were observed only in 55–65% of control patients. At 8 and 12 weeks, muscular electrophysiological function in the region dominated by the injured radial nerve was significantly better in the transplantation group than the control group. After cell transplantation, no immunological rejections were observed. These findings suggest that human umbilical cord mesenchymal stem cell-loaded amniotic membrane can be used for the repair of radial nerve injury.
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