Retinoic acid-induced differentiation of a nontumorigenic embryonal carcinoma cell mutant created through retroviral insertion.
Retinoic acid-induced differentiation of a nontumorigenic embryonal carcinoma cell mutant created through retroviral insertion.
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视黄酸诱导通过逆转录病毒插入产生的非致瘤性胚胎癌细胞突变体的分化。
DOI：
10.1016/0014-4827(92)90439-f
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发表时间：
1992
影响因子：
3.7
通讯作者：
McCormick,PJ
中科院分区：
文献类型：
--
作者：
Dietrich,J;Shin,HS;McCormick,PJ
A mutant embryonal carcinoma cell line, NR1-6, was created through retroviral insertion. We have previously reported that due to a single insertional event the mutant cell line is altered in regard to both its morphology and its tumorigenic capacity. We now report that this same cell line is also aberrant in its differentiative potential following exposure to the morphogen retinoic acid (RA). Unlike the parental NR1-0 cells, the NR1-6 cells apparently do not respond to RA by elaborating primitive endodermal derivatives in monolayer culture but rather appear morphologically to differentiate into mesodermal cells. This hypothesis is substantiated by the observation that RA treatment induces the transcription of both Endo A and B mRNA in parental but not mutant cells. No differences have been observed in the transcription of other RA sensitive markers such as c-myc, tissue plasminogen activator, collagen type IV, and laminin. In addition, the mutant cells are quantitatively much more sensitive to RA induction than are the parental cells, achieving full differentiation within 72 h of treatment with 10−10MRA. The parental cells, in contrast, will only differentiate at concentrations of 10−5or 10−6MRA, following 5 to 7 days of treatment. A spontaneous revertant cell line, which was isolated from an NR1-6 population and lacks the retroviral insert, is identical to the parental population in all parameters. Therefore, these data indicate that, in this case at least, a single genetic locus is involved in regulating both the qualitative and quantitative response of EC cells to RA-induced differentiation, as well as their morphology and tumorigenic potential.
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鉴定编码 Endo B 鼠细胞角蛋白的基因及其在小鼠非上皮细胞中的甲基化、稳定失活状态。
影响因子：
10.5
作者：
Oshima,RG;Trevor,K;Shevinsky,LH;Ryder,OA;Ceceña,G
通讯作者：
Ceceña,G
植入前小鼠胚胎中表达的 Endo B 细胞角蛋白的分子克隆和表征。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Singer,PA;Trevor,K;Oshima,RG
通讯作者：
Oshima,RG
对视黄酸反应改变的 F9 畸胎癌干细胞突变体的选择和表征。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wang,SY;Gudas,LJ
通讯作者：
Gudas,LJ
雏鸡肢芽中与视黄酸结合的细胞蛋白的空间分布
影响因子：
64.8
作者：
M. Maden;D. Ong;D. Summerbell;F. Chytil
通讯作者：
F. Chytil
DOI：
10.1016/s0021-9258(17)44608-4
发表时间：
1983-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Spiegelman;M. Frank;H. Green
通讯作者：
B. Spiegelman;M. Frank;H. Green