Odorant-binding proteins OBP57d and OBP57e affect taste perception and host-plant preference in Drosophila sechellia.
Odorant-binding proteins OBP57d and OBP57e affect taste perception and host-plant preference in Drosophila sechellia.
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DOI：
10.1371/journal.pbio.0050118
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发表时间：
2007-05
期刊：
影响因子：
9.8
通讯作者：
Fuyama Y
中科院分区：
文献类型：
--
作者：
Matsuo T;Sugaya S;Yasukawa J;Aigaki T;Fuyama Y
尽管其形态与黑腹果蝇物种复合体中的其他物种相似，但 D. sechellia 已对其寄主植物海巴戟（通常称为大溪地诺丽果）进化出独特的生理和行为适应。柑橘成熟果实的气味源自己酸和辛酸。 D. sechellia 被这两种脂肪酸吸引，而复合物中的其他物种则被排斥。在这里，利用黑腹果蝇缺陷突变体和黑腹果蝇之间的种间杂交，我们发现气味结合蛋白 57e (Obp57e) 基因与物种之间的行为差异有关。 Obp57e 和 Obp57d 基因敲除果蝇对己酸和辛酸的行为反应发生了改变。此外，从D. simulans和D. sechellia引入Obp57d和Obp57e将黑腹果蝇Obp57d/eKO蝇的产卵位点偏好转变为原始物种，证实了这些基因对D. sechellia向M. citrifolia特化的贡献。我们对参与宿主植物决定的基因的发现可能会导致进一步了解味觉、植物与食草动物相互作用的进化和物种形成的机制。大多数草食性昆虫专门捕食一种或几种寄主植物。了解这一专业化背后的过程和遗传学对整个生物学具有广泛的影响。塞舌尔果蝇 (Drosophila sechellia) 是塞舌尔特有的一种果蝇，专门以海巴戟 (Morinda citrifolia) 的成熟果实为食，巴戟天是一种热带植物，俗称大溪地诺丽果。尽管其他果蝇因其毒素而从不接近这种水果，但果蝇具有抵抗力，并且实际上被相同的毒素所吸引。黑腹果蝇 (D. sechellia) 是黑腹果蝇 (D. melanogaster) 的近亲，黑腹果蝇 (D. melanogaster) 是已建立的遗传学模式物种。通过比较黑腹果蝇和黑腹果蝇，我们发现编码气味结合蛋白的两个基因Obp57d和Obp57e不仅参与果蝇的味觉感知，而且还可以改变果蝇对水果中所含毒素的行为反应。通过了解昆虫的食物偏好如何由其基因决定，我们可以深入了解昆虫生活方式如何进化，并研究这种变化是否会导致新物种的形成。我们还可以开始了解如何通过改变昆虫对特定物质的偏好来操纵昆虫的行为。果蝇突变体和 D. 的杂交种Sechellia揭示了与行为差异有关的基因，这些差异使sechellia专门针对其宿主植物，这对于理解植物与草食动物的相互作用和物种形成具有重要意义
Despite its morphological similarity to the other species in the Drosophila melanogaster species complex, D. sechellia has evolved distinct physiological and behavioral adaptations to its host plant Morinda citrifolia, commonly known as Tahitian Noni. The odor of the ripe fruit of M. citrifolia originates from hexanoic and octanoic acid. D. sechellia is attracted to these two fatty acids, whereas the other species in the complex are repelled. Here, using interspecies hybrids between D. melanogaster deficiency mutants and D. sechellia, we showed that the Odorant-binding protein 57e (Obp57e) gene is involved in the behavioral difference between the species. D. melanogaster knock-out flies for Obp57e and Obp57d showed altered behavioral responses to hexanoic acid and octanoic acid. Furthermore, the introduction of Obp57d and Obp57e from D. simulans and D. sechellia shifted the oviposition site preference of D. melanogaster Obp57d/eKO flies to that of the original species, confirming the contribution of these genes to D. sechellia's specialization to M. citrifolia. Our finding of the genes involved in host-plant determination may lead to further understanding of mechanisms underlying taste perception, evolution of plant–herbivore interactions, and speciation. Most herbivorous insects specialize on one or a few host plants; understanding the processes and genetics underlying this specialization has broad implications across biology. Drosophila sechellia, a fruit fly endemic to the Seychelles, feeds exclusively on the ripe fruit of Morinda citrifolia, a tropical plant commonly known as Tahitian Noni. Although other fruit flies never approach this fruit because of its toxins, D. sechellia is resistant and is actually attracted by the same toxins. D. sechellia is a close relative of D. melanogaster, an established model species of genetics. By comparing D. melanogaster and D. sechellia, we revealed that two genes encoding odorant-binding proteins, Obp57d and Obp57e, are not only involved in the fruit fly's taste perception, but can also change the behavioral response of the flies to the toxins contained in the fruit. By knowing how an insect's food preference is determined by its genes, we can gain insight into how insect lifestyles evolve and investigate whether such changes can lead to the formation of new species. We can also begin to understand how to manipulate insects' behavior by changing their preference for particular substances. Hybrids ofDrosophila melanogaster mutants andD. sechellia reveal genes involved in the behavioral difference that makessechellia specialized to its host plant, with implications for understanding plant-herbivore interactions and speciation
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