Activation of TRPV4 Increases Neovascularization of Rat Prefabricated Flaps
Activation of TRPV4 Increases Neovascularization of Rat Prefabricated Flaps
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TRPV4 的激活增加大鼠预制皮瓣的新血管形成
DOI：
10.1055/s-0037-1607210
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发表时间：
2017-09
影响因子：
2.1
通讯作者：
Zan Tao
中科院分区：
文献类型：
--
作者：
Jinhong Bae;Zhichao Wang;Haizhou Li;Lin Lu;Qingxiong Yu;Qingfeng Li;Zan Tao
摘要 背景 新生血管形成不足是可导致预制皮瓣后续坏死的一个主要危险因素。近期证据表明，瞬时受体电位阳离子通道V亚族成员4（TRPV4）激活生长……
BackgroundInadequate neovascularization is a major risk factor that can lead to subsequent necrosis of prefabricated flaps. Recent evidence indicates that transient receptor potential cation channel, subfamily V, member 4 (TRPV4) activates growth and remodeling of collateral arteries in ischemia tissues by responding to elevated fluid shear stress (FSS). Therefore, we evaluated whether TRPV4 could increase neovascularization in prefabricated flaps in a rat model.MethodsRat prefabricated skin flaps were created by ligating the right femoral vascular pedicle and implanting it underneath abdominal flaps. Thirty-six male Sprague–Dawley rats were randomly assigned to three groups with different solutions injected subcutaneously in the implantation site around the pedicle: injected with normal saline as the control group; injected with 4α-Phorbol 12,13-didecanoate (4αPDD), a specific TRPV4 activator, as the 4αPDD group; or injected with ruthenium red (RR), a TRPV-blocker, as the RR group. Neovascularization was evaluated by laser speckle contrast imaging (FLPI), histological staining, and enzyme-linked immunosorbent assay (ELISA) within two weeks. Afterwards, the abdominal island flaps were completely elevated and sutured back. The flap viability and survival area were examined on day 7.ResultsA larger area of flap survival, higher capillary densities, and higher von Willebrand factor (vWF) expression were observed in the 4αPDD group in comparison to those in the other two groups. The secretion of vascular endothelial growth factor (VEGF), but not basic fibroblast growth factor (bFGF), was significantly elevated in the 4αPDD group.ConclusionActivation of TRPV4 using 4αPDD can significantly increase the survival of prefabricated flaps via neovascularization inducement, possibly through VEGF secretion enhancement. TRPV4 serves as a potential therapeutic neovascularization target in prefabricated flaps.
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影响因子：
2.9
作者：
Nelson MT;Bonev AD;Dalsgaard T;Santana LF;Scott J;Kotlikoff MI;Sonkusare SK
通讯作者：
Sonkusare SK
DOI：
10.1016/j.ejvs.2010.11.034
发表时间：
2011-05-01
影响因子：
5.7
作者：
Schierling, W.;Troidi, K.;Schmitz-Rixen, T.
通讯作者：
Schmitz-Rixen, T.
影响因子：
2.6
作者：
Junyan Yin;W. Kuebler
通讯作者：
Junyan Yin;W. Kuebler
影响因子：
2.1
作者：
Heng Xu;Zheng Zhang;Yimeng Xia;Zvi Steinberger;P. Min;Hua Li;Yahui Dai;Yixin Zhang
通讯作者：
Heng Xu;Zheng Zhang;Yimeng Xia;Zvi Steinberger;P. Min;Hua Li;Yahui Dai;Yixin Zhang
影响因子：
2.1
作者：
Heng Xu;S. Feng;Yimeng Xia;Zvi Steinberger;W. Xi;Hongwei Fang;Zhiwei Li;Yixin Xie;Yixin Zhang
通讯作者：
Heng Xu;S. Feng;Yimeng Xia;Zvi Steinberger;W. Xi;Hongwei Fang;Zhiwei Li;Yixin Xie;Yixin Zhang