An assessment of the resolution limitation due to radiation-damage in x-ray diffraction microscopy.
An assessment of the resolution limitation due to radiation-damage in x-ray diffraction microscopy.
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DOI：
10.1016/j.elspec.2008.10.008
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发表时间：
2009-03-01
影响因子：
1.9
通讯作者：
Starodub, D.
中科院分区：
文献类型：
--
作者：
Howells, M. R.;Beetz, T.;Chapman, H. N.;Cui, C.;Holton, J. M.;Jacobsen, C. J.;Kirz, J.;Lima, E.;Marchesini, S.;Miao, H.;Sayre, D.;Shapiro, D. A.;Spence, J. C. H.;Starodub, D.
X射线衍射显微镜（XDM）是一种新的X射线成像形式，目前正在几个第三代同步辐射X射线设施中实践。自该技术首次引入以来已经过去了九年，它在展示高分辨率三维成像方面取得了快速进展，并承诺在比透射电子显微镜成像更大的样品中具有几nm的分辨率。XDM的生命科学和材料科学应用都是预期的，并且预计分辨率的主要限制将是生命科学的辐射损伤和材料科学可用X射线源的相干功率。在本文中，我们解决的问题，辐射损伤的作用。我们使用的统计分析的基础上所谓的“剂量分割定理”的Hegerl和Hoppe计算所需的剂量，使一个单一的生命科学样本的图像XDM与给定的分辨率。我们发现，对于简单形状的物体所需的剂量尺度与分辨率的倒数四次方，并提出实验证据来支持这一发现。为了确定最大耐受剂量，我们收集了从文献中获取的大量数据以及我们自己的一些测量结果，这些数据涵盖了其他方法无法很好涵盖的分辨率范围。本研究的结论是，基于蛋白质和水之间的自然对比度和“玫瑰标准”图像质量，应该能够使用XDM以约10 nm的分辨率对冷冻水合生物样品进行成像。
X-ray diffraction microscopy (XDM) is a new form of x-ray imaging that is being practiced at several third-generation synchrotron-radiation x-ray facilities. Nine years have elapsed since the technique was first introduced and it has made rapid progress in demonstrating high-resolution three-dimensional imaging and promises few-nm resolution with much larger samples than can be imaged in the transmission electron microscope. Both life- and materials-science applications of XDM are intended, and it is expected that the principal limitation to resolution will be radiation damage for life science and the coherent power of available x-ray sources for material science. In this paper we address the question of the role of radiation damage. We use a statistical analysis based on the so-called “dose fractionation theorem” of Hegerl and Hoppe to calculate the dose needed to make an image of a single life-science sample by XDM with a given resolution. We find that for simply-shaped objects the needed dose scales with the inverse fourth power of the resolution and present experimental evidence to support this finding. To determine the maximum tolerable dose we have assembled a number of data taken from the literature plus some measurements of our own which cover ranges of resolution that are not well covered otherwise. The conclusion of this study is that, based on the natural contrast between protein and water and “Rose-criterion” image quality, one should be able to image a frozen-hydrated biological sample using XDM at a resolution of about 10 nm.
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影响因子：
3.6
作者：
FIENUP, JR
通讯作者：
FIENUP, JR
DOI：
10.1107/s0907444999016261
发表时间：
2000-03-01
影响因子：
2.2
作者：
Burmeister, WP
通讯作者：
Burmeister, WP
DOI：
10.1364/josaa.20.000040
发表时间：
2003-01-01
影响因子：
1.9
作者：
Elser, V
通讯作者：
Elser, V
影响因子：
2
作者：
GLAESER, RM;TAYLOR, KA
通讯作者：
TAYLOR, KA
DOI：
10.1515/zna-1976-1241
发表时间：
1976-01-01
影响因子：
1.8
作者：
HEGERL, R;HOPPE, W
通讯作者：
HOPPE, W