Genetic characterization of thymidine kinase from acyclovir-resistant and -susceptible herpes simplex virus type 1 isolated from bone marrow transplant recipients.
Genetic characterization of thymidine kinase from acyclovir-resistant and -susceptible herpes simplex virus type 1 isolated from bone marrow transplant recipients.
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从骨髓移植受者中分离出的抗阿昔洛韦和敏感的 1 型单纯疱疹病毒胸苷激酶的遗传特征。
DOI：
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发表时间：
2000
影响因子：
6.4
通讯作者：
D. Thouvenot
中科院分区：
文献类型：
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作者：
F. Morfin;G. Souillet;K. Bilger;T. Ooka;M. Aymard;D. Thouvenot
骨髓移植受者中出现对阿昔洛韦（Acy）耐药的单纯疱疹病毒（HSV）是一个主要问题。对分离自11例患者的7株Acy敏感型和11株Acy耐药型HSV-1进行了胸苷激酶（TK）的表型和遗传特征分析。总共检测到19个与Acy抗性无关但与TK基因多态性相关的氨基酸替换，包括5个先前未报道的突变。1例患者的Acy耐药菌株未出现与耐药相关的TK基因突变。5例患者（45%）的分离株携带点突变，导致可能与Acy耐药相关的氨基酸置换。在检测到的5个替换中，3个先前未报道（密码子51、83和175）。在5例患者（45%）的耐药分离株中检测到核苷酸插入或缺失;这些突变位于密码子92（1例受试者）和密码子146（4例受试者）的均聚物重复序列中。
Emergence of acyclovir (Acy)-resistant herpes simplex virus (HSV) is a major concern in bone marrow transplant recipients. Phenotypic and genetic characterization of thymidine kinase (TK) was done for 7 Acy-susceptible and 11 Acy-resistant HSV-1 isolated from 11 patients. In total, 19 amino acid substitutions were detected that were not related to Acy resistance but to TK gene polymorphism, including 5 mutations that have not been previously reported. The Acy-resistant strain from 1 patient presented no TK gene mutation related to resistance. Five patients (45%) had isolates that harbored point mutations leading to amino acid substitutions that could be associated with Acy resistance. Of the 5 substitutions detected, 3 have not been previously reported (codons 51, 83, and 175). A nucleotide insertion or deletion was detected in resistant isolates from 5 patients (45%); these mutations are located in homopolymer repeats at codon 92 (1 subject) and at codon 146 (4 subjects).
疱疹病毒脱氧胸苷激酶含有与猪腺苷酸激酶的磷酰结合富含精氨酸区域类似的位点；
DOI：
10.1099/0022-1317-71-12-2979
发表时间：
1990
期刊：
The Journal of general virology
影响因子：
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作者：
Balasubramaniam,NK;Veerisetty,V;Gentry,GA
通讯作者：
Gentry,GA