Resistance to wheat streak mosaic virus in transgenic wheat engineered with the viral coat protein gene
Resistance to wheat streak mosaic virus in transgenic wheat engineered with the viral coat protein gene
复制标题
用病毒外壳蛋白基因改造的转基因小麦对小麦条纹花叶病毒的抗性
DOI：
10.1023/a:1013944011049
复制
发表时间：
2002
影响因子：
3
通讯作者：
R. Qu
中科院分区：
文献类型：
--
作者：
E. Sivamani;C. Brey;L. Talbert;M. Young;W. E. Dyer;W. Kaniewski;R. Qu
Wheat (Triticum aestivum) plants were stably transformed with the coat protein (CP) gene of wheat streak mosaic virus (WSMV) by the biolistic method. Eleven independently transformed plant lines were obtained and five were analyzed for gene expression and resistance to WSMV. One line showed high resistance to inoculations of two WSMV strains. This line had milder symptoms and lower virus titer than control plants after inoculation. After infection, new growth did not show symptoms. The observed resistance was similar to the ‘recovery’ type resistance described previously using WSMV NIb transgene and in other systems. This line looked morphologically normal but had an unusually high transgene copy number (approximately 90 copies per 2C homozygous genome). Northern hybridization analysis indicated a high level of degraded CP mRNA expression. However, no coat protein expression was detected.
影响因子：
56.9
作者：
ABEL, PP;NELSON, RS;BEACHY, RN
通讯作者：
BEACHY, RN