Editing of glutamate receptor subunit B pre-mRNA in vitro by site-specific deamination of adenosine
Editing of glutamate receptor subunit B pre-mRNA in vitro by site-specific deamination of adenosine
复制标题
通过腺苷位点特异性脱氨作用体外编辑谷氨酸受体 B 亚基前 mRNA
作者：
Jing;P. Sklar;R. Axel;T. Maniatis
谷氨酸受体亚基B（GluR-B）前mRNA在单个腺苷残基处的编辑导致氨基酸变化，从而深刻地改变受体的电生理学特性1 -7。在这里，我们表明，GluR-B前体mRNA是有效和准确的编辑在体外，和编辑位点和下游内含子8中的序列之间的碱基对相互作用所需的底物识别。此外，我们直接证明，编辑的结果从腺苷转化为肌苷的酶促脱氨。这种GluR-B编辑活性的生物化学性质类似于双链RNA依赖性腺苷脱氨酶的生物化学性质9 -15，但RNA竞争和柱分级分离实验表明GluR-B编辑和脱氨酶活性是不同的。因此，GluR-B编辑酶可以含有腺苷脱氨酶或类似活性，以及将酶特异性靶向编辑位点的RNA识别亚基。
EDITING of the glutamate receptor subunit B (GluR-B) pre-mRNA at a single adenosine residue results in an amino-acid change that profoundly alters the electrophysiological properties of the receptor1–7. Here we show that the GluR-B pre-mRNA is efficiently and accurately edited in vitro, and that base-pair interactions between the editing site and a sequence in the downstream intron8 are required for substrate recognition. In addition, we directly demonstrate that editing results from the conversion of adenosine to inosine by enzymatic deamination. The biochemical properties of this GluR-B editing activity are similar to those of a double-stranded-RNA-dependent adenosine deaminase9–15, but RNA competition and column fractionation experiments indicate that the GluR-B editing and deaminase activities are distinct. Thus, the GluR-B editing enzyme may contain the adenosine deaminase, or a similar activity, and an RNA recognition subunit that specifically targets the enzyme to the editing site.
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DOI：
10.1016/s0021-9258(17)36857-6
发表时间：
1994-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
U. Kim;Tim L. Garner;Tamara Sanford;D. Speicher;J. Murray;K. Nishikura
通讯作者：
U. Kim;Tim L. Garner;Tamara Sanford;D. Speicher;J. Murray;K. Nishikura
DOI：
10.1073/pnas.91.24.11457
发表时间：
1994-11-22
影响因子：
11.1
作者：
KIM, U;WANG, Y;NISHIKURA, K
通讯作者：
NISHIKURA, K
影响因子：
56.9
作者：
MOORE, MJ;SHARP, PA
通讯作者：
SHARP, PA
DOI：
10.1073/pnas.86.8.2647
发表时间：
1989-04-01
影响因子：
11.1
作者：
WAGNER, RW;SMITH, JE;NISHIKURA, K
通讯作者：
NISHIKURA, K