Maternal stevioside supplementation improves intestinal immune function of chicken offspring potentially via modulating gut microbiota and down-regulating the promoter methylation level of suppressor of cytokine signaling 1 (SOCS1).
Maternal stevioside supplementation improves intestinal immune function of chicken offspring potentially via modulating gut microbiota and down-regulating the promoter methylation level of suppressor of cytokine signaling 1 (SOCS1).
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DOI：
10.1016/j.aninu.2022.06.002
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发表时间：
2022-09
期刊：
影响因子：
6.3
通讯作者：
Shi, Fangxiong
中科院分区：
文献类型：
--
作者：
Jiang, Jingle;Qi, Lina;Wei, Quanwei;Shi, Fangxiong
雏鸡的肠道免疫功能在生长早期受到限制。母亲的营养干预被认为会影响子代的先天免疫。本研究旨在探讨母体甜菊糖苷对仔鸡肠道免疫功能的影响。试验选用120只金茂黄羽种鸡，分别饲喂基础日粮和添加250 mg/kg甜菊糖苷的饲粮5周。在过去的一周里，每组收集了200枚种蛋进行孵化。孵化后，随机选择80只公鸡(每组40只)，饲喂相同的基础日粮28d，并孵化出90枚形状良好的种鸡受精蛋进行卵内注射试验。孵育7d后，注射20%甘油中的甜菊醇。结果表明，母猪饲喂甜菊糖苷能促进胚胎发育、空肠完整性和空肠隐窝的增殖(P<0.05)。母体甜菊糖苷还能提高仔鸡空肠细胞因子和内毒素耐受相关因子的固有转录水平(P<0.05)。在28岁时，母亲补充甜菊糖苷后的后代表现出更高的空肠分泌免疫球蛋白A和血清干扰素水平(P<0.05)。母亲补充甜菊糖诱导的乳酸菌丰度增加与肠道免疫相关因素呈正相关(P<0.05)。蛋内注射甜菊醇对孵化雏鸡的胚胎发育和肠道免疫功能均无影响(P>；0.05)。此外，母体补充甜菊糖苷可诱导细胞因子信号转导抑制因子1(SOCS1)启动子区的低甲基化。综上所述，母体甜菊糖苷可能通过调节肠道微生物区系和下调SOCS1启动子甲基化水平来改善仔鸡的肠道免疫功能。
The intestinal immune function of chickens is limited during the early growing stage. Maternal nutritional intervention has been suggested to affect the innate immunity of offspring. The present study aimed to investigate the effects of maternal stevioside supplementation on the intestinal immune function of chicken offspring. A total of 120 Jinmao yellow-feathered breeder hens were fed a basal diet or a diet supplemented with 250 mg/kg stevioside for 5 weeks. During the last week, 200 breeding eggs from each group were collected for incubation. After hatching, 80 male offspring (40 chickens from each group) were randomly selected and fed the same basal diet for 28 d. In addition, 90 well-shaped fertile eggs of non-treated breeder hens were incubated for the in ovo injection experiment. Steviol dissolved in 20% glycerol was injected at 7 d of incubation. The results showed that maternal stevioside supplementation could improve embryonic development, jejunal integrity and proliferation in the jejunal crypt (P < 0.05). Maternal stevioside supplementation could also increase the innate transcription levels of cytokines and endotoxin tolerance-related factors in the jejunum of chicken offspring (P < 0.05). At 28 d of age, the offspring following maternal stevioside supplementation exhibited higher jejunal secretory immunoglobulin A and serum interferons levels (P < 0.05). A higher abundance of Lactobacillales induced by maternal stevioside supplementation was positively correlated with intestinal immune-related factors (P < 0.05). The in ovo injection with steviol did not alter either embryonic development or intestinal immune function of hatching chickens (P > 0.05). Furthermore, maternal stevioside supplementation could induce hypo-methylation on the promoter region of suppressor of cytokine signaling 1 (SOCS1). In conclusion, maternal stevioside supplementation could improve the intestinal immune function of chicken offspring potentially via modulating the gut microbiota and down-regulating the promoter methylation level of SOCS1.
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影响因子：
4.4
作者：
Gong HZ;Lang WY;Lan HN;Fan YY;Wang TP;Chu QR;Wang JH;Li D;Zheng X;Wu M
通讯作者：
Wu M
影响因子：
3.7
作者：
Gou Z;Liu R;Zhao G;Zheng M;Li P;Wang H;Zhu Y;Chen J;Wen J
通讯作者：
Wen J
DOI：
10.4049/jimmunol.1700909
发表时间：
2018-01-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Alice AF;Kramer G;Bambina S;Baird JR;Bahjat KS;Gough MJ;Crittenden MR
通讯作者：
Crittenden MR
影响因子：
6.1
作者：
Geuns, JMC;Bruggeman, V;Buyse, JG
通讯作者：
Buyse, JG
影响因子：
6.1
作者：
Casas-Grajales, Sael;Ramos-Tovar, Erika;Muriel, Pablo
通讯作者：
Muriel, Pablo