Chromosomal rearrangements as a major mechanism in the onset of reproductive isolation in Saccharomyces cerevisiae.
Chromosomal rearrangements as a major mechanism in the onset of reproductive isolation in Saccharomyces cerevisiae.
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DOI：
10.1016/j.cub.2014.03.063
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发表时间：
2014-05-19
期刊：
影响因子：
9.2
通讯作者：
Schacherer, Joseph
中科院分区：
文献类型：
--
作者：
Hou, Jing;Friedrich, Anne;de Montigny, Jacky;Schacherer, Joseph
了解来自同一物种的个体之间生殖隔离如何演变的分子基础，为遗传分化模式以及物种形成的开始提供了有价值的见解。酿酒酵母由于其广泛的生态范围、高水平的遗传多样性和实验室可验证的有性繁殖而成为理想的模式。在S.在严格意义上的酿酒酵母复合体中，生殖隔离作用于合子后，并且可以归因于染色体重排、细胞核不相容性和抗重组;尽管由于新生物种的进一步分化，这些机制在物种形成的初期阶段的含义仍然不清楚。最近，几项研究评估了S.通过评估错配修复系统的作用或通过使用相同的初始遗传背景培养初期物种形成来鉴定酿酒酵母。然而，该物种的整体遗传多样性在很大程度上被忽视，也没有进行系统的评价。在这里，我们进行了第一次全物种的调查后合子生殖隔离在S。啤酒。我们从不同的生态位与参考菌株S288 c的60个自然分离物的样本，并确定了16例生殖隔离减少后代的活力从44%到86%不等。使用不同的映射策略，我们确定了大部分的所有菌株调查的相互易位，表明大规模的染色体重排可能发挥重要作用，在这个物种的生殖隔离的发病。
Understanding the molecular basis of how reproductive isolation evolves between individuals from the same species offers valuable insight into patterns of genetic differentiation as well as the onset of speciation. The yeast Saccharomyces cerevisiae constitutes an ideal model partly due to its vast ecological range, high level of genetic diversity and laboratory amendable sexual reproduction. Between S. cerevisiae and its sibling species in the Saccharomyces sensu stricto complex, reproductive isolation acts post-zygotically and could be attributed to chromosomal rearrangements, cyto-nuclear incompatibility and anti-recombination; although the implication of these mechanisms at the incipient stage of speciation remains unclear due to further divergence in the nascent species. Recently, several studies assessed the onset of intraspecific reproductive isolation in S. cerevisiae by evaluating the effect of the mismatch repair system or by fostering incipient speciation using the same initial genetic background. Nevertheless, the overall genetic diversity within this species was largely overlooked and no systematic evaluation has been performed. Here, we carried out the first species-wide survey for post-zygotic reproductive isolation in S. cerevisiae. We crossed 60 natural isolates sampled from diverse niches with the reference strain S288c, and identified 16 cases of reproductive isolation with reduced offspring viabilities ranging from 44% to 86%. Using different mapping strategies, we identified reciprocal translocations in a large fraction of all isolates surveyed, indicating that large-scale chromosomal rearrangements might play a major role to the onset of reproductive isolation in this species.
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影响因子：
9.8
作者：
Chou JY;Hung YS;Lin KH;Lee HY;Leu JY
通讯作者：
Leu JY
DOI：
10.1016/s0922-338x(98)80146-3
发表时间：
1998-01-01
期刊：
JOURNAL OF FERMENTATION AND BIOENGINEERING
影响因子：
--
作者：
Goto-Yamamoto, N;Kitano, K;Hara, S
通讯作者：
Hara, S
DOI：
10.1073/pnas.0510998103
发表时间：
2006-02-28
影响因子：
11.1
作者：
Heck, JA;Argueso, JL;Alani, E
通讯作者：
Alani, E
影响因子：
4.5
作者：
Greig, Duncan
通讯作者：
Greig, Duncan
影响因子：
64.8
作者：
Dettman, Jeremy R.;Sirjusingh, Caroline;Anderson, James B.
通讯作者：
Anderson, James B.