Characterization of human dermal sheath cells reveals CD36-expressing perivascular cells associated with capillary blood vessel formation in hair follicles.
Characterization of human dermal sheath cells reveals CD36-expressing perivascular cells associated with capillary blood vessel formation in hair follicles.
复制标题
人真皮鞘细胞的表征揭示了表达 CD36 的血管周围细胞与毛囊中毛细血管的形成相关。
DOI：
10.1016/j.bbrc.2019.06.146
复制
发表时间：
2019
影响因子：
3.1
通讯作者：
J. Kishimoto
中科院分区：
文献类型：
--
作者：
Yuzo Yoshida;T. Soma;J. Kishimoto
真皮鞘（DS）位于毛囊的最外边缘，包括这些毛囊的结缔组织鞘;已知DS细胞有助于毛发循环和毛囊新生。然而，DS细胞促进毛发形成的机制目前尚不清楚。我们研究了人类DS细胞在早期传代培养中的整体转录谱，并与人类毛乳头细胞（DP细胞）和真皮成纤维细胞进行了比较。血管相关基因在DS细胞中高度表达，并且多配体受体CD36在DS细胞中的表达显著高于DP细胞。整体成像技术的进一步分析表明，人生长期毛囊的DS中形成了致密的毛细血管网络，而休止期和退行期毛囊中观察到毛细血管消退。我们发现，CD36表达细胞存在于DS细胞群体中，但很少观察到DP细胞和成纤维细胞群体。此外，我们的研究结果表明，表达CD36的DS细胞可能参与血管生成。因此，我们得出结论，表达CD36的DS细胞可以调节毛囊中的毛细血管，与毛发周期有关。
Dermal sheath (DS) is located at the outermost border of hair follicles, comprising the connective tissue sheath of these follicles; DS cells are known to contribute to hair cycling and follicle neogenesis. However, the mechanisms by which DS cells contribute to hair formation are currently unclear. We investigated the global transcriptional profile of human DS cells in early passaged culture, compared with those of human dermal papilla cells (DP cells) and dermal fibroblasts. Vascular related genes were highly expressed in DS cells, and expression of the multi-ligand receptor, CD36, was significantly higher in DS cells than in DP cells. Further analyses with whole-mount imaging technique showed that dense networks of blood capillaries were formed in the DS of human anagen hair follicles, whereas regression of blood capillaries was observed in telogen and catagen hair follicles. We found that CD36-expressing cells were present in populations of DS cells, but were rarely observed in populations of DP cells and fibroblasts. Furthermore, our results indicated that CD36-expressing DS cells may participate in angiogenesis. Therefore, we concluded that CD36-expressing DS cells may modulate blood capillaries in hair follicles, in association with hair cycling.
影响因子：
15.9
作者：
Febbraio, M;Hajjar, DP;Silverstein, RL
通讯作者：
Silverstein, RL
影响因子：
11.8
作者：
Rahmani, Waleed;Abbasi, Sepideh;Biernaskie, Jeff
通讯作者：
Biernaskie, Jeff
影响因子：
15.9
作者：
Yano, K;Brown, LF;Detmar, M
通讯作者：
Detmar, M