Phosphorylation Of Platelet Actin-Binding Protein During Platelet Activation
Phosphorylation Of Platelet Actin-Binding Protein During Platelet Activation
复制标题
血小板激活过程中血小板肌动蛋白结合蛋白的磷酸化
DOI：
10.1055/s-0038-1652791
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发表时间：
1982
影响因子：
6.7
通讯作者：
J. Gerrard
中科院分区：
文献类型：
--
作者：
R. Carroll;J. Gerrard
我们跟踪了~(32)P标记的肌动蛋白结合蛋白作为血小板激活的函数。利用聚丙烯酰胺十二烷基硫酸钠凝胶电泳法分离总的血小板蛋白样品，我们发现凝血酶刺激后30到60秒肌动蛋白结合蛋白的标记增加了2到3倍。在40,000和20,000个表观分子量肽中观察到了较大的增加。通过纯化的肌动蛋白结合蛋白和洗涤剂提取活化的~(32)P标记的血小板制备的细胞骨架核心的共电泳法鉴定凝胶上的肌动蛋白结合蛋白。此外，这些细胞骨架核心表明，~(32)P标记的肌动蛋白结合蛋白与激活的血小板细胞骨架密切相关。在8分钟的时间过程中对肌动蛋白结合蛋白进行32P标记后发现，在聚集的血小板样本中，在3到5分钟之间发生了几乎初始水平的快速去磷酸化。对于表观分子量为20,000的多肽，也得到了类似的曲线。这种快速去磷酸化被证明依赖于在没有外部钙的情况下的血小板聚集，或者在缺乏对激活的聚集反应的促血栓形成的血小板中。这些结果表明，在激活过程中，肌动蛋白结合蛋白的磷酸化启动了它与血小板细胞骨架的联系。
We have followed the 32P-labelling of actin-binding protein as a function of platelet activation. Utilizing polyacrylamide sodium dodecyl sulfate gel electrophoresis to resolve total platelet protein samples we found 2 to 3 fold labelling increases in actin-binding protein 30 to 60 seconds after thrombin stimulation. Somewhat larger increases were observed for 40,000 and 20,000 apparent molecular weight peptides. The actin-binding protein was identified on the gels by coelectrophoresis of purified actin-binding protein as well as cytoskeletal cores prepared by detergent extraction of activated 32p-iabelled platelets. In addition, these cytoskeletal cores indicated that the 32P-labelled actin-binding protein was closely associated with the activated platelet's cytoskeleton. Following the 32P-labelling of actin-binding protein over an 8 minute time course revealed that in aggregating platelet samples rapid desphosphorylation to almost initial levels occurred between 3 and 5 minutes. A similar curve was obtained for the 20,000 apparent molecular weight peptide. This rapid dephosphorylation was shown to be dependent on platelet aggregation in the absence of external calcium or in thrombastenic platelets lacking the aggregation response to activation. These results suggest that phosphorylation of actin-binding protein initiates its association with the platelet cytoskeleton during activation.
细胞松弛素 B 与人血小板结合。
影响因子：
5.6
作者：
Zobel,CR;Jung,CY
通讯作者：
Jung,CY