Cytochalasin B binding by human platelets.
Cytochalasin B binding by human platelets.
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细胞松弛素 B 与人血小板结合。
DOI：
10.1002/jcp.1041130221
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发表时间：
1982
影响因子：
5.6
通讯作者：
Jung,CY
中科院分区：
文献类型：
--
作者：
Zobel,CR;Jung,CY
完整的人血小板与细胞松弛素B(Cb)的结合容量为10 0~120pmos Cb/mg蛋白或约7×10 4个分子/细胞，解离常数(Kd)在2×10−8~10−6M之间。高达85%的可饱和结合被10−5M细胞松弛素E(CE)取代。这种CE敏感的绑定与KD看起来也是异质的，类似于整体绑定的那些。然而，CE不敏感的结合表现为单一组分，Kd≌为4×10−7M。来自冷冻、解冻和洗涤细胞的可沉淀成分还与CB结合KD2.4×10−8到1.5×10−6M，总容量约为39pmos/mg蛋白质，仅占与完整细胞结合的配体的4%。这种CB结合的大部分(60%-80%)可被500mM的D-葡萄糖置换，KD值为1.5×10−6M，而只有10%-15%对CE敏感，KD值为2.4±10−8M。结果表明，95%的可饱和CB结合与胞浆有关，其中80%-85%对CE敏感，而只有3%的细胞结合是葡萄糖敏感的膜结合。如果与胞浆相关的CE敏感结合完全与肌动蛋白结合，则这种结合的化学计量比约为1-30个肌动蛋白单体，比CB与肌肉肌动蛋白结合大一个数量级。
Intact human platelets bind cytochalasin B (CB) with a capacity of 100– 120 p mols CB/mg protein or approximately 7 × 104molecules/cell and dissociation constants (KD) ranging from 2 × 10−8to 10−6M. Up to 85% of this saturable binding is displaced by 10−5M cytochalasin E (CE). This CE‐sensitive binding also appears heterogeneous with KDsimilar to those of the overall binding. The CE‐insensitive binding, however, appears as a single component with KD≌ 4 × 10−7M. The sedimentable constituents from frozen, thawed, and washed cells also bind CB with KDranging from 2.4 × 10−8to 1.5 × 10−6M and a total capacity of approximately 39 p mols/mg protein which accounts for only 4% of the ligand binding to the intact cell. The major portion (60–80%) of this CB binding is displaceable by 500 mM D‐glucose and has a KDof 1.5 × 10−6M, while only 10–15% is CE‐sensitive with a KDof 2.4 ± 10−8M. It is concluded that 95% of the saturable CB binding in platelets is associated with the cytosol of which 80–85% is sensitive to CE and that only 3% of the cellular binding is glucose sensitive, membrane‐associated binding. If the CE‐sensitive binding associated with the cytosol is entirely to actin, the stoichiometry of this binding is approximately one CB to 30 actin monomers, which is greater by an order of magnitude than that for CB binding to muscle actin.
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对照和利多卡因处理的人血小板的形状和细胞质丝。
影响因子：
20.3
作者：
V. Nachmias;J. Sullender;A. Asch
通讯作者：
A. Asch
DOI：
10.1083/jcb.68.3.602
发表时间：
1976-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Stossel TP;Hartwig JH
通讯作者：
Hartwig JH
慢性粒细胞白血病白细胞的肌动蛋白、肌球蛋白和肌动蛋白结合蛋白的相互作用。
影响因子：
15.9
作者：
L. Boxer;T. Stossel
通讯作者：
T. Stossel
DOI：
10.1016/0006-3002(61)90250-5
发表时间：
1961-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BETTEXGALLAND, M;LUSCHER, EF
通讯作者：
LUSCHER, EF
从人血小板中提取肌动球蛋白样蛋白
影响因子：
64.8
作者：
M. Bettex;E. Lüscher
通讯作者：
E. Lüscher