Experimental evolution of a bacteriophage virus reveals the trajectory of adaptation across a fecundity/longevity trade-off.
Experimental evolution of a bacteriophage virus reveals the trajectory of adaptation across a fecundity/longevity trade-off.
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DOI：
10.1371/journal.pone.0046322
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Brown SP
中科院分区：
文献类型：
--
作者：
Heineman RH;Brown SP
生活史理论试图解释有机体如何领导他们的生活，平衡繁殖和生存的相互冲突的需求。在这里，我们跟踪的基因组和表型进化的噬菌体病毒T7在一个假设的繁殖力/寿命的限制。我们使T7适应具有挑战性的生存环境（6 M尿素）。我们进化的菌株显示繁殖体存活率的显着改善，再加上繁殖力的显着损失（宿主细胞上的生长速率降低）。然而，对尿素的抗性增加并没有普遍增加对温度胁迫的抗性，这突出表明繁殖体的耐久性是环境依赖性的。先前的比较研究预测繁殖体抗性的变化将由衣壳蛋白的变化或基因缺失介导。相反，我们发现内部核心蛋白基因（6.7和16）的点突变是我们进化菌株尿素抗性增加的原因。在6.7和16突变出现之前，一组独特的5点突变在衰减之前达到超过20%的患病率，表明适应过程中的负上位相互作用。我们的研究结果表明，寄生虫可以适应特定的传播环境，这种适应可能会对随后利用宿主细胞的能力造成成本，可能会限制持久的寄生虫降低毒力。
Life history theory attempts to account for how organisms lead their lives, balancing the conflicting demands of reproduction and survival. Here, we track the genomic and phenotypic evolution of the bacteriophage virus T7 across a postulated fecundity/longevity constraint. We adapted T7 to a challenging survival environment (6M urea). Our evolved strain displayed a significant improvement in propagule survival, coupled with a significant loss of fecundity (reduced growth rate on host cells). However, the increased resistance to urea did not generalise to increased resistance against temperature stress, highlighting that propagule durability is environment dependent. Previous comparative studies predicted that changes in propagule resistance would be mediated by changes in capsid proteins or gene deletions. In contrast, we found that point mutations in internal core protein genes (6.7 and 16) were responsible for the increased urea resistance of our evolved strain. Prior to the emergence of the 6.7 and 16 mutations, a distinct set of 5-point mutations peaked at over 20% prevalence before attenuating, suggestive of negative epistatic interactions during adaptation. Our results illustrate that parasites can adapt to specific transmission environments, and that this adaptation can impose costs on the subsequent ability to exploit host cells, potentially constraining durable parasites to lower virulence.
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影响因子：
2.9
作者：
Heineman, Richard H.;Springman, Rachael;Bull, James J.
通讯作者：
Bull, James J.
影响因子：
3.7
作者：
BLEICHRODT, JF;BLOK, J;ABKOUDE, ERB
通讯作者：
ABKOUDE, ERB
影响因子：
3.7
作者：
Kemp, P;Garcia, LR;Molineux, IJ
通讯作者：
Molineux, IJ
影响因子：
2.9
作者：
Alizon, S;van Baalen, M
通讯作者：
van Baalen, M
DOI：
10.1073/pnas.0708559104
发表时间：
2007-10-30
影响因子：
11.1
作者：
Fraser, Christophe;Hollingsworth, T. Deirdre;Hanage, William P.
通讯作者：
Hanage, William P.