Expression of cyclins A and D and p21(waf1/cip1) proteins in renal cell cancer and their relation to clinicopathological variables and patient survival.
Expression of cyclins A and D and p21(waf1/cip1) proteins in renal cell cancer and their relation to clinicopathological variables and patient survival.
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DOI：
10.1038/sj.bjc.6690634
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发表时间：
1999-08
影响因子：
8.8
通讯作者：
Kosma, VM
中科院分区：
文献类型：
--
作者：
Aaltomaa, S;Lipponen, P;Ala-Opas, M;Eskelinen, M;Syrjänen, K;Kosma, VM
我们研究了118例肾细胞癌，分析细胞周期蛋白A和D1和p21（waf 1/cip 1）的表达，以及它们与临床和组织病理学参数以及临床预后的关系。正常肾组织中不表达细胞周期蛋白A、D1和细胞周期蛋白依赖性激酶抑制剂p21（waf 1/cip 1）。cyclin D1和p21（waf 1/cip 1）的染色信号总是在细胞核中，但cyclin A也在肿瘤细胞的胞浆中表达。细胞周期蛋白A、细胞周期蛋白D1和p21（waf 1/cip 1）阳性肿瘤细胞的平均（范围）分数分别为2.2%（范围0-20%）、23.3%（范围0-90%）和6.8%（范围0-70%）。cyclin A的表达与肿瘤的静脉浸润、高核分级、高核分裂率、高Ki-67和高PCNA表达有关（P均≤ 0.006）。cyclin D1的表达与年龄> 65岁、核分级低、p53高表达有关（均P ≤ 0.05）。p21（waf 1/cip 1）与cyclin D1呈负相关（P = 0.011）。细胞周期蛋白A预测了整个研究组（P = 0.0014）、T1-4/N 0 -2/M0（P = 0.0007）和T1-2/N 0/M0肿瘤（P = 0.0007）的生存率。细胞周期蛋白A也是T1-4/N 0/M0（P = 0.0027）肿瘤（P = 0.0007）无病生存期的有力预测因子。细胞周期蛋白D1和p21（waf 1/cip 1）与任何组的生存率或无病生存率均无显著相关。在所有病例中，影响预后的独立因素有远处转移（相对危险度（RR）5.16，P < 0.001）、T细胞分类（RR 2.68，P < 0.001）、Ki-67表达（RR 1.02，P = 0.026）和cyclin A表达（RR 1.12，P = 0.001）。T1-4/N 0/M0期肿瘤的独立预测因子为T分类（RR 2.67，P = 0.001）和细胞周期蛋白A（RR 1.21，P < 0.001），T1-2/N 0/M0期肿瘤的唯一显著预测因子为细胞周期蛋白A（RR 1.19，P = 0.0002）。在肾细胞癌中，细胞周期蛋白A是一个强大的和独立的预后因素，在疾病的所有临床阶段，而细胞周期蛋白D1和p21（waf 1/cip 1）没有预后价值。© 1999癌症研究运动
We have studied 118 renal cell carcinomas to analyse the expressions of cyclins A and D1 and p21(waf1/cip1), and their relationship to clinical and histopathological parameters as well as to clinical outcome. Cyclins A and D1 and cyclin-dependent kinase inhibitor p21(waf1/cip1) were not expressed in normal renal tissue. Staining signals of cyclin D1 and p21(waf1/cip1) were always nuclear but cyclin A was also expressed in the cytoplasm of the tumour cells. The mean (range) fractions of cyclin A, cyclin D1 and p21(waf1/cip1)-positive tumour cells were 2.2% (range 0–20%), 23.3% (range 0–90%) and 6.8% (range 0–70%) respectively. The expression of cyclin A was related to venous invasion, high nuclear grade, high mitotic rate, high Ki-67 and high PCNA expressions (P ≤ 0.006 for all). The expression of cyclin D1 was linked with age over 65 years, low nuclear grade and high p53 expression (P ≤ 0.05 for all). An inverse correlation was present between p21(waf1/cip1) and cyclin D1 (P = 0.011). Cyclin A predicted survival in the entire study group (P = 0.0014), in T1–4/N0–2/M0 (P = 0.0007) and in T1–2/N0/M0 tumours (P = 0.0007). Cyclin A was also a powerful predictor of disease-free survival in T1–4/N0/M0 (P = 0.0027) tumours (P = 0.0007). Cyclin D1 and p21(waf1/cip1) were not significantly related to survival or disease-free survival in any of the groups. In the entire material the independent prognostic factors were the presence of distant metastases (relative risk (RR) 5.16, P < 0.001), T category (RR 2.68, P < 0.001), Ki-67 expression (RR 1.02, P = 0.026) and cyclin A expression (RR 1.12, P = 0.001). The independent predictors in T1–4/N0/M0 tumours were T-category (RR 2.67, P = 0.001) and cyclin A (RR 1.21, P < 0.001), and in T1–2/N0/M0 tumours the only significant predictor was cyclin A (RR 1.19, P = 0.0002). In renal cell carcinoma, cyclin A is a powerful and independent prognostic factor in all clinical stages of the disease, whereas cyclin D1 and p21(waf1/cip1) have no prognostic value. © 1999 Cancer Research Campaign
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DOI：
10.1046/j.1464-410x.1997.03399.x
发表时间：
1997-02-01
期刊：
BRITISH JOURNAL OF UROLOGY
影响因子：
--
作者：
Byrne, RL;Horne, CHW;Hamdy, FC
通讯作者：
Hamdy, FC
影响因子：
23.4
作者：
Lipponen, P;Aaltomaa, S;Kosma, VM
通讯作者：
Kosma, VM
影响因子：
6.4
作者：
LIPPONEN, P;ESKELINEN, M;SYRJANEN, K
通讯作者：
SYRJANEN, K
影响因子：
2.1
作者：
Papandreou, CN;Bogenrieder, T;Nanus, DM
通讯作者：
Nanus, DM
影响因子：
2.8
作者：
HAAPASALO, H;PESONEN, E;COLLAN, Y
通讯作者：
COLLAN, Y