Ion channel expression in the developing enteric nervous system.
Ion channel expression in the developing enteric nervous system.
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DOI：
10.1371/journal.pone.0123436
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
McKeown SJ
中科院分区：
文献类型：
--
作者：
Hirst CS;Foong JP;Stamp LA;Fegan E;Dent S;Cooper EC;Lomax AE;Anderson CR;Bornstein JC;Young HM;McKeown SJ
肠神经系统起源于神经嵴源性细胞（ENCC），其沿胚胎肠向尾部沿着迁移。采用PCR芯片、RT-PCR和免疫组织化学方法研究了胚胎小鼠ENCC中离子通道的表达。许多离子通道，包括氯离子、钙离子、钾离子和钠离子通道，在E11.5时已经被ENCC表达。在E11.5和E14.5之间，许多离子通道基因的表达增加，这与ENCC迁移和肠神经元的神经突的第一次延伸相一致。以往的研究表明，多种离子通道调节神经突起的延伸和迁移的许多细胞类型。一系列氯离子或钙离子通道的药理学抑制对体外培养外植体中ENCC迁移或轴突发生没有影响。非选择性钾通道抑制剂TEA和4-AP延缓了ENCC迁移和轴突发生，但仅在导致细胞死亡的浓度下。总之，当ENCC在肠道定植时，大量离子通道表达，但我们没有发现ENCC迁移或神经突生成需要氯、钙或钾通道活性的证据。许多离子通道可能参与肠神经元电兴奋性的发展。
The enteric nervous system arises from neural crest-derived cells (ENCCs) that migrate caudally along the embryonic gut. The expression of ion channels by ENCCs in embryonic mice was investigated using a PCR-based array, RT-PCR and immunohistochemistry. Many ion channels, including chloride, calcium, potassium and sodium channels were already expressed by ENCCs at E11.5. There was an increase in the expression of numerous ion channel genes between E11.5 and E14.5, which coincides with ENCC migration and the first extension of neurites by enteric neurons. Previous studies have shown that a variety of ion channels regulates neurite extension and migration of many cell types. Pharmacological inhibition of a range of chloride or calcium channels had no effect on ENCC migration in cultured explants or neuritogenesis in vitro. The non-selective potassium channel inhibitors, TEA and 4-AP, retarded ENCC migration and neuritogenesis, but only at concentrations that also resulted in cell death. In summary, a large range of ion channels is expressed while ENCCs are colonizing the gut, but we found no evidence that ENCC migration or neuritogenesis requires chloride, calcium or potassium channel activity. Many of the ion channels are likely to be involved in the development of electrical excitability of enteric neurons.
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