Topological similarity between the 2μm plasmid partitioning locus and the budding yeast centromere: evidence for a common evolutionary origin?
Topological similarity between the 2μm plasmid partitioning locus and the budding yeast centromere: evidence for a common evolutionary origin?
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2μm 质粒分配位点和芽殖酵母着丝粒之间的拓扑相似性：共同进化起源的证据？
DOI：
10.1042/bst20120224
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发表时间：
2013
影响因子：
3.9
通讯作者：
Soumitra Sau
中科院分区：
文献类型：
--
作者：
M. Jayaram;Keng;Chien;Chu‐Chun Huang;Yen;Soumitra Sau
酿酒酵母自私质粒2 dna m环的分离位点stb对这种具有类似染色体稳定性的多拷贝染色体外μ元件的繁殖是必不可少的。STB的功能不仅需要宿主编码蛋白Rep1和Rep2，还需要质粒编码的分割蛋白Rep1和Rep2。以Rep1和Rep2依赖的方式与STB相关的宿主因子也与着丝粒相互作用，并在染色体分离中发挥重要作用。它们包括粘附素复合体和着丝粒特异的组蛋白H3变异体Cse4。酿酒酵母的基因定义点着丝粒与真核生物中分布更为广泛的表观遗传特定的区域着丝粒截然不同。酿酒酵母着丝粒的特别小的尺寸和染色体分离因子与STB的关联增加了这两个分割基因座之间进化联系的可能性。最近的实验揭示了酿酒酵母着丝粒和STB在体内功能状态下不同寻常的正超级卷曲，支持了它们可能从祖先的质粒分割位点进化而来的概念。
The partitioning locus STB of the selfish plasmid, the 2μm circle, of Saccharomyces cerevisiae is essential for the propagation of this multi-copy extra-chromosomal DNA element with nearly chromosome-like stability. The functional competence of STB requires the plasmid-coded partitioning proteins Rep1 and Rep2 as well as host-coded proteins. Host factors that associate with STB in a Rep1- and Rep2-dependent manner also interact with centromeres, and play important roles in chromosome segregation. They include the cohesin complex and the centromere-specific histone H3 variant Cse4. The genetically defined point centromere of S. cerevisiae differs starkly from the much more widespread epigenetically specified regional centromeres of eukaryotes. The particularly small size of the S. cerevisiae centromere and the association of chromosome segregation factors with STB raise the possibility of an evolutionary link between these two partitioning loci. The unusual positive supercoiling harboured by the S. cerevisiae centromere and STB in vivo in their functional states, unveiled by recent experiments, bolsters the notion of their potential descent from an ancestral plasmid partitioning locus.
影响因子：
16
作者：
Huang, J;Hsu, JM;Laurent, BC
通讯作者：
Laurent, BC
出芽和裂殖酵母中着丝粒的结构和功能。
DOI：
--
发表时间：
1990
期刊：
The New biologist
影响因子：
--
作者：
Carbon,J;Clarke,L
通讯作者：
Clarke,L
影响因子：
13.8
作者：
Sekulic, Nikolina;Black, Ben E.
通讯作者：
Black, Ben E.