In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions.
In vitro ubiquitination and degradation of bacteria-purified human histone H2B by the nucleoli fractions.
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核仁组分对细菌纯化的人组蛋白 H2B 的体外泛素化和降解
DOI：
10.1016/j.xpro.2021.100601
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发表时间：
2021-06-18
期刊：
影响因子：
--
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Li S;Zhang G;Liu Y;Sun Q;Yang L;Dong J;Zhang B;Chen S
组蛋白是染色质的主要组成部分，组蛋白的蛋白水平显著影响染色质的组装。在这里，我们描述了详细的协议组蛋白H2B纯化从细菌和核仁组分和细胞质和核质组分的分离。最后，在体外泛素化和降解H2B不同的细胞组分进行了描述。有关本方案使用和执行的完整详细信息，请参阅。纯化核仁、细胞质和核质的方案通过这些细胞组分进行的体外H2B降解和泛素化测定通过核仁组分进行的细菌组蛋白H2B的体外泛素化和降解组蛋白是染色质的主要组分，并且组蛋白的蛋白质水平显著影响染色质组装。在这里，我们描述了详细的协议组蛋白H2B纯化从细菌和核仁组分和细胞质和核质组分的分离。最后，在体外泛素化和降解H2B不同的细胞组分进行了描述。
Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described. For complete details on the use and execution of this protocol, please refer to. Protocols to purify nucleoli, cytoplasm, and nucleoplasm In vitro H2B degradation and ubiquitination assays by these cellular components In vitro ubiquitination and degradation of bacterial histone H2B by the nucleoli fractions Histones are the main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. Here, we describe detailed protocols for histone H2B purification from bacteria and for the separation of nucleolar fractions and cytoplasmic and nucleoplasmic fractions. Finally, the in vitro ubiquitination and degradation of H2B by distinct cellular fractions are described.
H2B 单泛素化对 Warburg 效应的表观遗传调控
影响因子：
12.4
作者：
Jing, Yuan-Ya;Cai, Feng-Feng;Chen, Su
通讯作者：
Chen, Su
核仁充当组蛋白 H2B 蛋白降解的隔室
影响因子：
5.8
作者：
Liu Y;Wang Y;Yang L;Sun F;Li S;Wang Y;Zhang GA;Dong T;Zhang LL;Duan W;Zhang X;Cui W;Chen S
通讯作者：
Chen S