The nucleolus functions as the compartment for histone H2B protein degradation.
The nucleolus functions as the compartment for histone H2B protein degradation.
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核仁充当组蛋白 H2B 蛋白降解的隔室
DOI：
10.1016/j.isci.2021.102256
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发表时间：
2021-04-23
期刊：
影响因子：
5.8
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Liu Y;Wang Y;Yang L;Sun F;Li S;Wang Y;Zhang GA;Dong T;Zhang LL;Duan W;Zhang X;Cui W;Chen S
组蛋白是染色质的主要组成部分，组蛋白的蛋白水平对染色质的组装有重要影响。然而，组蛋白水平是如何调节的，特别是组蛋白是否以及如何降解，在很大程度上还不清楚。在这里，我们发现组蛋白H2 B主要通过蛋白酶体介导的途径降解，H2 B的赖氨酸-120位点是其K48连接的多聚泛素化和降解所必需的。此外，降解受损的H2 BK 120 R突变体显示出增加的核仁定位，并且蛋白酶体的抑制导致野生型H2 B的核仁分布升高，这与H2 BK 120 R突变体的核仁分布相似。更重要的是，核仁组分可以在体外泛素化和降解纯化的H2 B，这表明核仁，除了其典型的作用，调节核糖体生成和蛋白质翻译，可能与H2 B降解。因此，这些研究结果揭示了一种新的机制，在其中的核仁相关的蛋白酶体途径参与的H2 B降解的调节。组蛋白H2 B可以在赖氨酸120残基处被多聚泛素化。组蛋白H2 B的降解是通过泛素化-蛋白酶体途径实现的。核仁调节组蛋白H2 B的蛋白质降解生物化学;细胞生物学
Histones are main components of chromatin, and the protein levels of histones significantly affect chromatin assembly. However, how histone protein levels are regulated, especially whether and how histones are degraded, is largely unclear. Here, we found that histone H2B is mainly degraded through the proteasome-mediated pathway, and the lysine-120 site of H2B is essential for its K48-linked polyubiquitination and degradation. Moreover, the degradation-impaired H2BK120R mutant shows an increased nucleolus localization, and inhibition of the proteasome results in an elevated nucleolus distribution of wild-type H2B, which is similar to that of H2BK120R mutants. More importantly, the nucleolus fractions can ubiquitinate and degrade the purified H2B in vitro, suggesting that the nucleolus, in addition to its canonical roles regulating ribosome genesis and protein translation, likely associates with H2B degradation. Therefore, these findings revealed a novel mechanism for the regulation of H2B degradation in which a nucleolus-associated proteasome pathway is involved. Histone H2B can be polyubiquitinated at the lysine 120 residue The degradation of histone H2B is achieved via the ubiquitination-proteasome pathway The nucleolus regulates the protein degradation of histone H2B Biochemistry; Cell Biology
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DOI：
10.1016/j.bbamcr.2013.02.028
发表时间：
2014-01
影响因子：
5.1
作者：
Bassermann, Florian;Eichner, Ruth;Pagano, Michele
通讯作者：
Pagano, Michele
H2B 单泛素化和 p53 对铁死亡的表观遗传调控
影响因子：
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作者：
Wang, Yufei;Yang, Lu;Chen, Su
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Chen, Su
Def 磷酸化通过 Def 募集 Calpain3 调节核仁 p53 周转和细胞周期进展
影响因子：
9.8
作者：
Guan Y;Huang D;Chen F;Gao C;Tao T;Shi H;Zhao S;Liao Z;Lo LJ;Wang Y;Chen J;Peng J
通讯作者：
Peng J
影响因子：
56.9
作者：
Dion, Michael F.;Kaplan, Tommy;Rando, Oliver J.
通讯作者：
Rando, Oliver J.
影响因子：
5.3
作者：
Wu, Jiaxue;Huen, Michael S. Y.;Yu, Xiaochun
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Yu, Xiaochun