Renal IGF-1 mRNA levels are enhanced following unilateral nephrectomy in immature but not adult rats.
Renal IGF-1 mRNA levels are enhanced following unilateral nephrectomy in immature but not adult rats.
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未成熟大鼠单侧肾切除后肾脏 IGF-1 mRNA 水平升高，但成年大鼠则不然。
DOI：
10.1210/endo-128-5-2660
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发表时间：
1991
期刊：
影响因子：
4.8
通讯作者：
D. Leroith
中科院分区：
文献类型：
--
作者：
S. Mulroney;A. Haramati;C. Roberts;D. Leroith
成年大鼠单侧肾切除术（UNX）后IGF-1基因表达的增加存在争议。在这项研究中，我们研究了UNX对IGF-1基因表达的影响是否存在发育差异。未成熟（23天）和成年（4个月）Wistar大鼠进行假手术或左UNX，并在24或48小时后处死。使用溶液杂交/RNA酶保护测定法测定左（对照）和右（代偿）肾中的IGF-1 mRNA水平。UNX后48小时，成年大鼠的残肾生长了20 +/- 1%（P <0.05），未成年大鼠的残肾生长了69 +/- 5%（P <0.05）。在UNX后24或48小时，成人代偿肾中IGF-1 mRNA水平未增加。相比之下，UNX后24和48小时未成熟大鼠的肾脏外显子1 IGF-1 mRNA水平平均增加4倍（P <0.05），外显子2 mRNA水平平均增加3倍（P <0.05）。因此，这些研究结果表明，UnX对IGF-1基因表达的影响存在年龄依赖性差异，并提供了第一个证据表明，在未成熟大鼠单侧肾切除术后IGF-1基因表达增加。
The increase in IGF-1 gene expression following unilateral nephrectomy (UNX) in adult rats is controversial. In this study we have examined whether developmental differences exist in the effect of UNX on IGF-1 gene expression. Immature (23 days) and adult (4 months) Wistar rats underwent a sham operation or left UNX, and were sacrificed 24 or 48 hrs later. IGF-1 mRNA levels were determined in left (control) and right (compensated) kidneys using solution hybridization/RNase protection assays. By 48 hrs post-UNX, remnant kidneys had grown 20 +/- 1% in adult rats (P less than 0.05), and 69 +/- 5% in immature rats (P less than 0.05). IGF-1 mRNA levels were not increased in the adult compensated kidneys at either 24 or 48 hrs post-UNX. In contrast, kidneys from immature rats 24 and 48 hrs post-UNX had an average 4-fold increase (P less than 0.05) in exon 1 IGF-1 mRNA levels, and an average 3-fold increase (P less than 0.05) in exon 2 mRNA levels. Thus, these findings suggest that there is an age-dependent difference in the effects of UnX on IGF-1 gene expression, and provide the first evidence that IGF-1 gene expression increases following unilateral nephrectomy in immature rats.
肾肥大期间胰岛素样生长因子 I 表达的双重调节。
DOI：
10.1152/ajprenal.1989.257.2.f252
发表时间：
1989
期刊：
The American journal of physiology
影响因子：
--
作者：
Lajara,R;Rotwein,P;Bortz,JD;Hansen,VA;Sadow,JL;Betts,CR;Rogers,SA;Hammerman,MR
通讯作者：
Hammerman,MR