Binding of the SARS-CoV-2 spike protein to glycans.
Binding of the SARS-CoV-2 spike protein to glycans.
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SARS-CoV-2 刺突蛋白与聚糖的结合
DOI：
10.1016/j.scib.2021.01.010
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发表时间：
2021-06-30
期刊：
影响因子：
18.9
通讯作者：
Tan Z
中科院分区：
文献类型：
--
作者：
Hao W;Ma B;Li Z;Wang X;Gao X;Li Y;Qin B;Shang S;Cui S;Tan Z
本研究系统地研究了 SARS-CoV-2 和 SARS-CoV、MERS-CoV 的亚基和刺突 (S) 蛋白与硫酸乙酰肝素 (HS) 和含唾液酸聚糖的结合。我们的结果表明，所有测试的蛋白质分子都能以硫酸化依赖性方式与 HS 结合，并且未检测到与唾液酸残基的结合。总的来说，这项工作表明 HS 结合可能是这些冠状病毒附着到宿主细胞的一般机制，并支持 HS 在感染和开发针对这些病毒的抗病毒药物中的潜在重要性。严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的流行已在世界范围内造成大量死亡。为了对抗它，有必要更好地了解病毒如何感染宿主细胞。感染通常始于病毒附着在细胞表面聚糖上，如硫酸乙酰肝素 (HS) 和含唾液酸的糖脂/糖蛋白。在本研究中，我们检查并比较了 SARS-CoV-2、SARS-CoV 和中东呼吸系统疾病 (MERS)-CoV 的亚基和刺突 (S) 蛋白与这些聚糖的结合。我们的结果表明，S 蛋白和亚基可以以硫酸化依赖性方式与 HS 结合，并且未检测到与唾液酸残基的结合。总的来说，这项工作表明 HS 结合可能是这些冠状病毒附着到宿主细胞的一般机制，并支持 HS 在感染和开发针对这些病毒的抗病毒药物中的潜在重要性。
This study systematically investigated the binding of the subunits and spike (S) proteins of SARS-CoV-2 and SARS-CoV, MERS-CoV to heparan sulfate (HS) and sialic acid-containing glycans. Our results revealed that all the tested protein molecules can bind to HS in a sulfation-dependent manner and no binding with sialic acid residues was detected. Overall, this work suggests that HS binding may be a general mechanism for the attachment of these coronaviruses to host cells, and supports the potential importance of HS in infection and in the development of antiviral agents against these viruses. The pandemic of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a high number of deaths in the world. To combat it, it is necessary to develop a better understanding of how the virus infects host cells. Infection normally starts with the attachment of the virus to cell-surface glycans like heparan sulfate (HS) and sialic acid-containing glycolipids/glycoproteins. In this study, we examined and compared the binding of the subunits and spike (S) proteins of SARS-CoV-2, SARS-CoV, and Middle East respiratory disease (MERS)-CoV to these glycans. Our results revealed that the S proteins and subunits can bind to HS in a sulfation-dependent manner and no binding with sialic acid residues was detected. Overall, this work suggests that HS binding may be a general mechanism for the attachment of these coronaviruses to host cells, and supports the potential importance of HS in infection and in the development of antiviral agents against these viruses.
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