Transgenic Eimeria tenella Expressing Profilin of Eimeria maxima Elicits Enhanced Protective Immunity and Alters Gut Microbiome of Chickens
Transgenic Eimeria tenella Expressing Profilin of Eimeria maxima Elicits Enhanced Protective Immunity and Alters Gut Microbiome of Chickens
复制标题
表达巨型艾美耳球虫 Profilin 的转基因柔嫩艾美耳球虫可增强鸡的保护性免疫力并改变其肠道微生物组
DOI：
10.1128/iai.00888-17
复制
发表时间：
2018-07
影响因子：
3.1
通讯作者：
Suo Xun
中科院分区：
文献类型：
--
作者：
Tang Xinming;Suo Jingxia;Li Chao;Du Mengze;Wang Chaoyue;Hu D;an;Duan Chunhui;Lyu Yanli;Liu Xianyong;Suo Xun
球虫病是世界上危害最严重的畜禽疾病之一。通过遗传操作提高疫苗株的免疫原性，接种活寄生虫抗球虫疫苗将是控制种鸡和蛋鸡球虫病的最佳手段，即使在快速生长的肉鸡中也是如此。球虫病是世界上危害最严重的畜禽疾病之一。通过遗传操作提高疫苗株的免疫原性，接种活寄生虫抗球虫疫苗将是控制种鸡和蛋鸡球虫病的最佳手段，即使在快速生长的肉鸡中也是如此。来自顶复门寄生虫的Profilin是小鼠中第一个Toll样受体11（TLR 11）和TLR 12的分子定义的配体，并且是潜在的分子佐剂。本研究构建了表达巨型艾美耳球虫（Eimeria maxima）profilin蛋白的转基因柔嫩艾美耳球虫（Eimeria tenella）株（Et-EmPro），并研究了EmPro对鸡球虫免疫原性的影响。tenella。我们发现，与野生型相比，用转基因艾美耳球虫寄生虫免疫，引起更大的寄生虫抗原特异性细胞介导的免疫，其特征是分泌干扰素γ（IFN-γ）的淋巴细胞的数量增加。转基因寄生虫也诱导了较好的抗E. tenella的挑战比野生型。此外，用转基因寄生虫免疫的鸟的粪便微生物组的多样性与野生型免疫的鸟的微生物组的多样性不同，表明艾美球虫与鸡的肠道微生物组的相互作用。我们的结果显示增强的免疫原性E。通过使用EmPro作为来源于最具免疫原性的亲缘物种的分子佐剂，对柔嫩艾美耳球虫进行免疫接种，代表了在使用艾美耳球虫作为表达分子佐剂和潜在的其他病原体抗原的疫苗平台开发下一代球虫病疫苗方面向前迈出的一大步，所述分子佐剂和其他病原体抗原不仅针对球虫病，而且还针对其他传染病。
Coccidiosis is one of the most serious diseases of livestock and birds in the world. Vaccination with live-parasite anticoccidial vaccines with genetic manipulation improving the immunogenicity of vaccine strains would be the best means for controlling coccidiosis in breeder and layer stocks, even in fast-growing broilers. ABSTRACT Coccidiosis is one of the most serious diseases of livestock and birds in the world. Vaccination with live-parasite anticoccidial vaccines with genetic manipulation improving the immunogenicity of vaccine strains would be the best means for controlling coccidiosis in breeder and layer stocks, even in fast-growing broilers. Profilin from apicomplexan parasites is the first molecularly defined ligand for Toll-like receptor 11 (TLR11) and TLR12 in mice and is a potential molecular adjuvant. Here, we constructed a transgenic Eimeria tenella line (Et-EmPro) expressing the profilin of Eimeria maxima, the most immunogenic species of chicken coccidia, and evaluated the adjuvant effects of EmPro on the immunogenicity of E. tenella. We found that immunization with the transgenic Eimeria parasites, compared with the wild type, elicited greater parasite antigen-specific cell-mediated immunity, characterized by increased numbers of interferon gamma (IFN-γ)-secreting lymphocytes. The transgenic parasite also induced better protective immunity against E. tenella challenge than the wild type. In addition, the diversity of the fecal microbiome of the birds immunized with the transgenic parasite differed from that of the microbiome of the wild-type-immunized birds, indicating interactions of Eimeria with the gut microbiome of chickens. Our results showing enhanced immunogenicity of E. tenella by use of EmPro as a molecular adjuvant derived from the most immunogenic affinis species represent a large step forward in the development of the next generation of coccidiosis vaccines using Eimeria as a vaccine platform expressing molecular adjuvants and potentially other pathogen antigens against not only coccidiosis but also other infectious diseases.
登录
查看更多内容
影响因子：
3.7
作者：
Macdonald SE;Nolan MJ;Harman K;Boulton K;Hume DA;Tomley FM;Stabler RA;Blake DP
通讯作者：
Blake DP
影响因子：
30.3
作者：
Benson A;Pifer R;Behrendt CL;Hooper LV;Yarovinsky F
通讯作者：
Yarovinsky F
影响因子：
2.1
作者：
Zuoyong Zhou;K. Nie;Qingzhou Huang;Kai Li;Yingying Sun;R. Zhou;Zhiying Wang;Shijun Hu
通讯作者：
Zuoyong Zhou;K. Nie;Qingzhou Huang;Kai Li;Yingying Sun;R. Zhou;Zhiying Wang;Shijun Hu
影响因子：
6.7
作者：
Blake DP;Billington KJ;Copestake SL;Oakes RD;Quail MA;Wan KL;Shirley MW;Smith AL
通讯作者：
Smith AL
DOI：
10.4049/jimmunol.181.12.8478
发表时间：
2008-12-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Yarovinsky F;Hieny S;Sher A
通讯作者：
Sher A