Effects of Eimeria tenella infection on chicken caecal microbiome diversity, exploring variation associated with severity of pathology.
Effects of Eimeria tenella infection on chicken caecal microbiome diversity, exploring variation associated with severity of pathology.
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DOI：
10.1371/journal.pone.0184890
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Blake DP
中科院分区：
文献类型：
--
作者：
Macdonald SE;Nolan MJ;Harman K;Boulton K;Hume DA;Tomley FM;Stabler RA;Blake DP
艾美耳球虫属引起肠道疾病球虫病，尤其是在家禽中。虽然球虫病对动物健康和福利的直接影响是明确的，但其对肠道微生物群的影响以及对鸡健康和生产的旁观者效应在很大程度上仍然未知，可能除了产气荚膜梭菌（坏死性肠炎）。本研究使用16 S rRNA扩增子测序评估了Cobb 500肉鸡中存在或不存在定义的柔嫩艾美耳球虫攻毒感染时盲肠微生物组的组成和结构。通过盲肠病变评分和与鉴定的不同病变评分相关的微生物变化来量化个体鸡中临床球虫病的严重程度。继E. tenella感染盲肠微生物组内分类群的多样性基本保持稳定。然而，感染引起的一些微生物类群的丰度显着变化。最大的变化被检测到在鸟类显示严重的盲肠病理;属于肠杆菌科的类群增加，而芽孢杆菌目和乳杆菌目的类群减少与病变严重程度的变化。显着不同的配置文件中也检测到感染的鸟类，保持无症状（病变评分0），分类群属于拟杆菌减少和乳酸杆菌增加。鉴定了许多来自梭菌目的差异分类群，在艾美耳球虫感染的动物中，一些数量增加，另一些数量减少。结果支持这样的观点，即与艾美耳球虫感染相关的盲肠微生物组生态失调有助于疾病病理学，并可能成为减轻球虫病对家禽生产力和福利影响的干预目标。这项工作表明，E. tenella感染对某些盲肠细菌的丰度具有显著影响，在病变评分类别之间检测到显著差异，这强调了在研究E. tenella和家禽肠道微生物组。
Eimeria species cause the intestinal disease coccidiosis, most notably in poultry. While the direct impact of coccidiosis on animal health and welfare is clear, its influence on the enteric microbiota and by-stander effects on chicken health and production remains largely unknown, with the possible exception of Clostridium perfringens (necrotic enteritis). This study evaluated the composition and structure of the caecal microbiome in the presence or absence of a defined Eimeria tenella challenge infection in Cobb500 broiler chickens using 16S rRNA amplicon sequencing. The severity of clinical coccidiosis in individual chickens was quantified by caecal lesion scoring and microbial changes associated with different lesion scores identified. Following E. tenella infection the diversity of taxa within the caecal microbiome remained largely stable. However, infection induced significant changes in the abundance of some microbial taxa. The greatest changes were detected in birds displaying severe caecal pathology; taxa belonging to the order Enterobacteriaceae were increased, while taxa from Bacillales and Lactobacillales were decreased with the changes correlated with lesion severity. Significantly different profiles were also detected in infected birds which remained asymptomatic (lesion score 0), with taxa belonging to the genera Bacteroides decreased and Lactobacillus increased. Many differential taxa from the order Clostridiales were identified, with some increasing and others decreasing in abundance in Eimeria-infected animals. The results support the view that caecal microbiome dysbiosis associated with Eimeria infection contributes to disease pathology, and could be a target for intervention to mitigate the impact of coccidiosis on poultry productivity and welfare. This work highlights that E. tenella infection has a significant impact on the abundance of some caecal bacteria with notable differences detected between lesion score categories emphasising the importance of accounting for differences in caecal lesions when investigating the relationship between E. tenella and the poultry intestinal microbiome.
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DOI：
10.5713/ajas.15.0026
发表时间：
2015-09
期刊：
Asian-Australasian journal of animal sciences
影响因子：
--
作者：
Choi KY;Lee TK;Sul WJ
通讯作者：
Sul WJ
影响因子：
4
作者：
Clark EL;Macdonald SE;Thenmozhi V;Kundu K;Garg R;Kumar S;Ayoade S;Fornace KM;Jatau ID;Moftah A;Nolan MJ;Sudhakar NR;Adebambo AO;Lawal IA;Álvarez Zapata R;Awuni JA;Chapman HD;Karimuribo E;Mugasa CM;Namangala B;Rushton J;Suo X;Thangaraj K;Srinivasa Rao AS;Tewari AK;Banerjee PS;Dhinakar Raj G;Raman M;Tomley FM;Blake DP
通讯作者：
Blake DP
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
64.5
作者：
Goodrich JK;Di Rienzi SC;Poole AC;Koren O;Walters WA;Caporaso JG;Knight R;Ley RE
通讯作者：
Ley RE